Title
Roux-en-Y gastric bypass surgery changes fungal and bacterial microbiota in morbidly obese patients-A pilot study
Author
Steinert, R.E.
Rehman, A.
Souto Lima, E.J.
Agamennone, V.
Schuren, F.H.J.
Gero, D.
Schreiner, P.
Vonlanthen, R.
Ismaeil, A.
Tzafos, S.
Hosa, H.
Vetter, D.
Misselwitz, B.
Bueter, M.
Publication year
2020
Abstract
The Roux-en-Y gastric bypass (RYGB) remains the most effective treatment for morbidly obese patients to lower body weight and improve glycemic control. There is recent evidence that the mycobiome (fungal microbiome) can aggravate disease severity in a number of diseases including inflammatory bowel disease (IBD), irritable bowel syndrome (IBS) and hepatitis; moreover, a dysbiotic fungal microbiota has been reported in the obese. We characterized fungal and bacterial microbial composition in fecal samples of 16 morbidly obese patients before and three months after RYGB surgery and compared with nine healthy controls. We found that RYGB surgery induced a clear alteration in structure and composition of the gut fungal and bacterial microbiota. Beta diversity analysis revealed significant differences in bacterial microbiota between obese patients before surgery and healthy controls (P < 0.005) and a significant, unidirectional shift in RYGB patients after surgery (P < 0.001 vs. before surgery). In contrast, there was no significant difference in fungal microbiota between groups but individually specific changes after RYGB surgery. Interestingly, RYGB surgery induced a significant reduction in fungal alpha diversity namely Chao1, Sobs, and Shannon diversity index (P
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http://resolver.tudelft.nl/uuid:c33d0b54-edbd-4bc6-b233-d640bd296caf
DOI
https://doi.org/10.1371/journal.pone.0236936
TNO identifier
878725
Source
Plos One, 15 (15), e0236936
Document type
article