Title
Decreased interleukin-10 and increased interleukin- 12p40 mRNA are associated with disease activity and characterize different disease stages in multiple sclerosis
Author
van Boxel-Dezaire, A.H.H.
Hoff, S.C.J.
van Oosten, B.W.
Verweij, C.L.
Dräger, A.M.
Adèr, H.J.
van Houwelingen, J.C.
Barkhof, F.
Polman, C.H.
Nagelkerken, L.
TNO Preventie en Gezondheid
Publication year
1999
Abstract
It has been shown that proinflammatory and antiinflammatory cytokines correlate with disease activity in multiple sclerosis (MS). To establish whether such correlations depend on the disease stage, we assessed in a longitudinal fashion the expression of interleukin (IL)-12 (p40 and p35), tumor necrosis factor- 1/4 , interferon-γ, and IL-10 mRNA by competitive polymerase chain reaction in unstimulated peripheral blood mononuclear cells of relapsing-remitting (RR) and secondary progressive (SP) MS patients, in relation to monthly clinical and magnetic resonance imaging monitoring. MS patients had increased levels of IL-12p40 and decreased levels of IL-10 mRNA compared with controls; this difference was most pronounced in SP patients. Both RR and SP patients had increased levels of IL-12p40 mRNA compared with controls during the development of active lesions. Moreover, in RR MS an increase was found before relapse. IL-12p35 mRNA was decreased in both groups, and in relation to disease activity it showed a pattern different from IL-12p40 mRNA. In RR MS, IL-10 mRNA was low 4 weeks before magnetic resonance imaging activity and 6 weeks before relapse; a significant increase to normal levels was noted when active lesions became apparent. In contrast, SP patients showed low IL-10 mRNA levels constitutively, suggesting that IL- 10 plays an important role in the control of disease progression. Chemicals/CAS: Interleukin-10, 130068-27-8; Interleukin-12, 187348-17-0; RNA, Messenger
Subject
Health
Adult
Brain
Female
Humans
Interleukin-10
Interleukin-12
Longitudinal Studies
Magnetic Resonance Imaging
Male
Middle Aged
Multiple Sclerosis
Polymerase Chain Reaction
Recurrence
RNA, Messenger
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DOI
https://doi.org/10.1002/1531-8249(199906)45:6<695::aid-ana3>3.0.co;2-r
TNO identifier
235059
ISSN
0364-5134
Source
Annals of Neurology, 45 (6), 695-703
Document type
article