Print Email Facebook Twitter Expression profiling of colon cancer cell lines and colon biopsies: Towards a screening system for potential cancer-preventive compounds Title Expression profiling of colon cancer cell lines and colon biopsies: Towards a screening system for potential cancer-preventive compounds Author van Erk, M.J. Krul, C.A.M. Caldenhoven, E. Stierum, R.H. Peters, W.H. Woutersen, R.A. van Ommen, B. TNO Kwaliteit van Leven TNO Voeding Publication year 2005 Abstract Interest in mechanisms of colon cancer prevention by food compounds is strong and research in this area is often performed with cultured colon cancer cells. In order to assess utility for screening of potential cancer-preventive (food) compounds, expression profiles of 14 human cell lines derived from colonic tissue were measured using cDNA microarrays with 4000 genes and compared with expression profiles in biopsies of human colon tumours and normal tissue. Differences and similarities in the gene expression profiles of the cell lines were analysed by clustering and principal component analysis (PCA). Cytoskeleton genes and immune response genes are two functional classes of genes that contributed to the differences between the cell lines. A subset of 72 colon cancer-specific genes was identified by comparing expression profiles in human colon biopsies of tumour tissue and normal tissue. A separation of the cell lines based on the tumour stage of the original adenocarcinoma was observed after PCA of expression data of the subset of colon cancer-specific genes in the cell lines. The results of this study may be useful in the ongoing research into mechanisms of cancer prevention by dietary components. © 2005 Lippincott Williams & Wilkins. Subject HealthToxicology and Applied PharmacologyCancer preventionCell linesColon biopsiesColon cancerExpression profilingAPC proteinBeta cateninComplementary DNACyclooxygenase 2Protein p53AdultCancer cell cultureCancer preventionCancer researchCancer screeningCancer stagingClinical articleColon biopsyColon cancerControlled studyData analysisDNA microarrayFemaleGene expressionGenetic analysisHumanHuman cellHybridizationImmune response geneMaleMutationNucleotide sequenceOncogene c mycOncogene rasPrincipal component analysisPriority journalProtein expressionRNA isolationTumor suppressor geneAdenocarcinomaAdultBiopsyCell Line, TumorColonic NeoplasmsFemaleGastric MucosaGene Expression ProfilingGene Expression Regulation, NeoplasticGenes, NeoplasmGenetic ScreeningHumansMaleMiddle AgedMutation, MissenseNeoplasm ProteinsOligonucleotide Array Sequence AnalysisRNA, NeoplasmTumor Markers, Biological To reference this document use: http://resolver.tudelft.nl/uuid:bde4484d-12c7-4687-9c44-0907df3df7cd DOI https://doi.org/10.1097/01.cej.0000174781.51883.21 TNO identifier 238734 ISSN 0959-8278 Source European Journal of Cancer Prevention, 14 (5), 439-457 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.