Print Email Facebook Twitter Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry Title Application of matrix-assisted laser desorption/ionization to on-line aerosol time-of-flight mass spectrometry Author Stowers, M.A. van Wuijckhuijse, A.L. Marijnissen, J.C.M. Scarlett, B. van Baar, B.L.M. Kientz, Ch.E. Publication year 2000 Abstract Matrix-assisted laser desorption/ionization (MALDI) mass spectra were obtained from single biological aerosol particles using an aerosol time-of- flight mass spectrometer (ATOFMS). The inlet to the ATOFMS was coupled with an evaporation/condensation flow cell that allowed the aerosol to be coated with matrix material as the sampled stream entered the spectrometer. Mass spectra were generated from aerosol composed either of gramicidin-S or erythromycin, two small biological molecules, or from aerosolised spores of Bacillus subtilis var niger. Three different matrices were used: 3- nitrobenzyl alcohol, picolinic acid and sinapinic acid. A spectrum of gramicidin-S was generated from approximately 250 attomoles of material using a molar ratio of 3-nitrobenzyl alcohol to analyte of approximately 20:1. A single peak, located at 1224 Da, was obtained from the bacterial spores. The washing liquid and extract solution from the spores were analyzed using electrospray mass spectrometry and subsequent MS/MS product ion experiments. This independent analysis suggests that the measured species represents part of the B. subtilis peptidoglycan. The on-line addition of matrix allows quasi-real-time chemical analysis of individual, aerodynamically sized particles, with an overall system residence time of less than 5 seconds. These results suggest that a MALDIATOFMS can provide nearly real-time identification of biological aerosols. (C) 2000 John Wiley and Sons, Ltd. Subject Benzyl alcohol derivativeErythromycinGramicidin SPicolinic acidSinapic acidAerosolArticleBacillus subtilisBacterial sporeChemical analysisEvaporationMass spectrometryNonhumanPrecipitation To reference this document use: http://resolver.tudelft.nl/uuid:bca0547e-d352-4db0-9eb2-702f5a927827 DOI https://doi.org/10.1002/(sici)1097-0231(20000530)14:10<829::aid-rcm951>3.0.co;2-3 TNO identifier 235359 ISSN 0951-4198 Source Rapid Communications in Mass Spectrometry, 14 (10), 829-833 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.