Print Email Facebook Twitter Suitability of PER.C6 cells to generate epidemic and pandemic influenza vaccine strains by reverse genetics Title Suitability of PER.C6 cells to generate epidemic and pandemic influenza vaccine strains by reverse genetics Author Koudstaal, W. Hartgroves, L. Havenga, M. Legastelois, I. Ophorst, C. Siewerts, M. Zuijdgeest, D. Vogels, R. Custers, J. de Boer-Luijtze, E. de Leeuw, O. Cornelissen, L. Goudsmit, J. Barclay, W. TNO Kwaliteit van Leven Publication year 2009 Abstract Reverse genetics, the generation of influenza viruses from cDNA, presents a rapid method for creating vaccine strains. The technique necessitates the use of cultured cells. Due to technical and regulatory requirements, the choice of cell lines for production of human influenza vaccines is limited. PER.C6® cells, among the most extensively characterized and documented cells, support growth of all influenza viruses tested to date, and can be grown to high densities in large bioreactors in the absence of serum or micro carriers. Here, the suitability of these cells for the generation of influenza viruses by reverse genetics was investigated. A range of viruses reflective of vaccine strains was rescued exclusively using PER.C6 cells by various transfection methods, including an animal component-free procedure. Furthermore, a whole inactivated vaccine carrying the HA and NA segments of A/HK/156/97 (H5N1) that was both rescued from and propagated on PER.C6 cells, conferred protection in a mouse model. Thus PER.C6 cells provide an attractive platform for generation of influenza vaccine strains via reverse genetics. © 2009 Elsevier Ltd. All rights reserved. Subject BiologyBiomedical ResearchAnimalsAntibodies, ViralCell Culture TechniquesCell LineFemaleHemagglutination Inhibition TestsHumansInfluenza A Virus, H1N1 SubtypeInfluenza A Virus, H3N2 SubtypeInfluenza A Virus, H5N1 SubtypeInfluenza VaccinesInfluenza, HumanMiceMice, Inbred BALB CPlaque AssayReassortant VirusesSeverity of Illness IndexSurvival AnalysisTransfection To reference this document use: http://resolver.tudelft.nl/uuid:b96d387c-7c45-4536-b0a2-6fe4a0c33ae1 DOI https://doi.org/10.1016/j.vaccine.2009.02.033 TNO identifier 285080 Source Vaccine, 27 (19), 2588-2593 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.