Print Email Facebook Twitter Effect of protein charge on the generation of aggregation-prone conformers Title Effect of protein charge on the generation of aggregation-prone conformers Author Broersen, K. Weijers, M. de Groot, J. Hamer, R.J. de Jongh, H.H.J. TNO Kwaliteit van Leven Publication year 2007 Abstract This study describes how charge modification affects aggregation of ovalbumin, thereby distinguishing the role of conformational and electrostatic stability in the process. Ovalbumin variants were engineered using chemical methylation or succinylation to obtain a range of protein net charge from -1 to -26. Charge modification significantly affected the denaturation temperature. From urea-induced equilibrium denaturation studies, it followed that unfolding proceeded via an intermediate state. However, the heat-induced denaturation process could still be described as a two-state irreversible unfolding transition, suggesting that the occurrence of an intermediate has no influence on the kinetics of unfolding. By monitoring the aggregation kinetics, the net charge was found not to be discriminative in the process. It is concluded that the dominant factor determining ovalbumin aggregation propensity is the rate of denaturation and not electrostatic repulsive forces. © 2007 American Chemical Society. Subject NutritionFood technologyChemical modificationConformationsDenaturationElectrostaticsMethylationProteinsTemperatureAggregation kineticsConformersElectrostatic repulsive forcesOvalbuminBiomoleculesovalbuminarticleconformational transitioncontrolled studydenaturationelectricityheatingmethylationnonhumanphysical chemistrypriority journalprotein aggregationprotein engineeringsurface chargeAnimalsElectrostaticsHeatKineticsOvalbuminProtein DenaturationProtein FoldingUrea To reference this document use: http://resolver.tudelft.nl/uuid:b2f932da-743a-48f3-a90a-189005e7c419 DOI https://doi.org/10.1021/bm0612283 TNO identifier 239952 ISSN 1525-7797 Source Biomacromolecules, 8 (5), 1648-1656 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.