Title
Gaining Insight into Microbial Physiology in the Large Intestine: A Special Role for Stable Isotopes
Author
de Graaf, A.A.
Venema, K.
TNO Kwaliteit van Leven
Contributor
Poole, R.K. (editor)
Publication year
2008
Abstract
The importance of the human large intestine for nutrition, health, and disease, is becoming increasingly realized. There are numerous indications of a distinct role for the gut in such important issues as immune disorders and obesity-linked diseases. Research on this long-neglected organ, which is colonized by a myriad of bacteria, is a rapidly growing field that is currently providing fascinating new insights into the processes going on in the colon, and their relevance for the human host. This review aims to give an overview of studies dealing with the physiology of the intestinal microbiota as it functions within and in interaction with the host, with a special focus on approaches involving stable isotopes. We have included general aspects of gut microbial life as well as aspects specifically relating to genomic, proteomic, and metabolomic studies. A special emphasis is further laid on reviewing relevant methods and applications of stable isotope-aided metabolic flux analysis (MFA). We argue that linking MFA with the '-omics' technologies using innovative modeling approaches is the way to go to establish a truly integrative and interdisciplinary approach. Systems biology thus actualized will provide key insights into the metabolic regulations involved in microbe-host mutualism and their relevance for health and disease. © 2008 Elsevier Ltd. All rights reserved. Chemicals / CAS: butyric acid, 107-92-6, 156-54-7, 461-55-2; Fatty Acids, Volatile; Radioisotopes; Radiopharmaceuticals
Subject
Biology
Biomedical Research
Butyric acid
Prebiotic agent
Stable isotope
Bacterial phenomena and functions
Biomedicine
Ecosystem
Energy balance
Genome
Genomics
Immune system
Innate immunity
Intestine flora
Large intestine
Metabolic stress
Metabolomics
Microbial metabolism
Microorganism
Nonhuman
Obesity
PH
Proteomics
Review
Bacterial Physiology
Fatty Acids, Volatile
Genomics
Humans
Intestine, Large
Isotope Labeling
Proteomics
Radioisotopes
Radiopharmaceuticals
To reference this document use:
http://resolver.tudelft.nl/uuid:9fb5ac52-5790-4bfc-8c9a-97ea12af990d
DOI
https://doi.org/10.1016/s0065-2911(07)53002-x
TNO identifier
240139
ISBN
0123737133
ISSN
0065-2911
Source
Advances in Microbial Physiology, 53
Document type
conference paper