Title
Clearance of coagulation factor VIII in very low-density lipoprotein receptor knockout mice
Author
Bovenschen, N.
van Dijk, K.W.
Havekes, L.M.
Mertens, K.
van Vlijmen, B.J.M.
Gaubius Instituut TNO
Publication year
2004
Abstract
Low-density lipoprotein receptor-related protein (LRP) contributes to factor VIII (FVIII) catabolism in vivo. Besides LRP, FVIII also interacts with very low-density lipoprotein receptor (VLDLR) in vitro. We investigated the physiological role of VLDLR in FVIII catabolism, using knockout mouse models for VLDLR and LRP, alone and in combination. VLDLR-/- mice displayed normal plasma FVIII, whereas VLDLR-/- LRP- double-knockout mice had slightly increased FVIII compared with LRP-deficient mice. Remarkably, VLDLR deficiency slightly accelerated FVIII clearance. Adenovirus-mediated overexpression of VLDLR did not lower plasma FVIII in LRP-deficient mice. We conclude that VLDLR does not act in concert with LRP in FVIII clearance in vivo. Chemicals / CAS: blood clotting factor 8, 9001-27-8; cholesterol, 57-88-5; Cre recombinase, EC 2.7.7.-; Factor VIII, 9001-27-8; Integrases, EC 2.7.7.-; LDL-Receptor Related Proteins; Receptors, LDL; VLDL receptor
Subject
Biology
Biomedical Research
Clearance
Factor VIII
Knockout mice
Low-density lipoprotein receptor-related protein
Very low-density lipoprotein receptor
blood clotting factor 8
cholesterol
triacylglycerol
very low density lipoprotein receptor
animal experiment
article
catabolism
cholesterol blood level
controlled study
knockout mouse
male
mouse
nonhuman
plasma clearance
priority journal
triacylglycerol blood level
Animals
Factor VIII
Integrases
LDL-Receptor Related Proteins
Mice
Mice, Knockout
Mice, Transgenic
Receptors, LDL
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DOI
https://doi.org/10.1111/j.1365-2141.2004.05093.x
TNO identifier
237975
ISSN
0007-1048
Source
British Journal of Haematology, 126 (5), 722-725
Document type
article