Print Email Facebook Twitter Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy Title Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy Author Mittag, D. Akkerdaas, J. Ballmer-Weber, B.K. Vogel, L. Wensing, M. Becker, W.M. Koppelman, S.J. Knulst, A.C. Helbling, A. Hefle, S.L. van Ree, R. Vieths, S. TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO Publication year 2004 Abstract We recently described patients with soybean allergy mainly mediated by cross-reactivity to birch pollen allergens. A majority of those patients were reported to have peanut allergy. We sought to study the occurrence of peanut allergy in patients allergic to birch pollen and characterized the Bet v 1-homologous peanut allergen Ara h 8. Recombinant Ara h 8 was cloned with degenerated primers and expressed in Escherichia coli. Nine Swiss and 11 Dutch patients with peanut and birch pollen allergy and a positive double-blind, placebo-controlled food challenge result to peanut were investigated for IgE reactivity to birch pollen and purified peanut allergens and cross-reactivity between birch and peanut. Ara h 8 stability against digestion and roasting was assessed by means of RAST inhibition. The IgE cross-linking potency of Ara h 8 was tested on the basis of basophil histamine release. During double-blind, placebo-controlled food challenge, all patients experienced symptoms in the oral cavity, progressing to more severe symptoms in 40% of patients. CAP-FEIA detected recombinant (r) Ara h 8-specific IgE in 85%. IgE binding to Ara h 8 was inhibited by Bet v 1 in peanut extract immunoblotting and in RAST inhibition. In EAST inhibition recombinant rAra h 8 inhibited IgE binding to peanut in 4 of 7 tested patient sera. Antipeanut response was dominated by Ara h 8 in 12 of 17 tested patients. Furthermore, our results demonstrate a low stability of Ara h 8 to roasting and no stability to gastric digestion. Basophil histamine release with rAra h 8 was more than 20% in 5 of 7 tested sera. Peanut allergy might be mediated in a subgroup of our patients by means of cross-reaction of Bet v 1 with the homologous peanut allergen Ara h 8. Subject NutritionFood technologyAra h 8Birch pollen-related food allergyDiagnosisDouble-blindGly m 4LegumesOral allergy syndromePeanut allergyPlacebo-controlled food challengeRecombinant allergenAllergenAra h 8 allergenBet v 1 allergenHistamineUnclassified drugAdultAmino acid sequenceAntigen antibody reactionAntigen specificityBasophilBirchBlood analysisClinical articleControlled studyCross linkingCross reactionDigestionFemaleHistamine releaseHumanImmunoblottingMaleMolecular cloningMouth cavityPeanut allergyPollen allergyPriority journalProvocation testAdolescentAdultAllergensAmino Acid SequenceBasophilsBetulaCross ReactionsDouble-Blind MethodFemaleHistamine ReleaseHumansImmunoglobulin EMaleMolecular Sequence DataPeanut HypersensitivityPepsin APollenRecombinant Proteins To reference this document use: http://resolver.tudelft.nl/uuid:9d730eda-9c1f-4f77-8b20-a6f13ab5f3e7 DOI https://doi.org/10.1016/j.jaci.2004.09.014 TNO identifier 238247 ISSN 0091-6749 Source Journal of Allergy and Clinical Immunology, 114 (6), 1410-1417 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.