Title
Pyrosequencinq analysis of the oral microflora of healthy adults
Author
Keijser, B.J.F.
Zaura, E.
Huse, S.M.
van der Vossen, J.M.B.M.
Schuren, F.H.J.
Montijn, R.C.
ten Gate, J.M.
Crielaard, W.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
A good definition of commensal microflora and an understanding of its relation to health are essential in preventing and combating disease. We hypothesized that the species richness of human oral microflora is underestimated. Saliva and supragingival plaque were sampled from 71 and 98 healthy adults, respectively. Amplieons from the V6 hypervariable region of the small-subunit ribosomal RNA gene were generated by PCR, pooled into saliva and plaque pools, and sequenced by means of the Genome Sequencer 20 system at 454 Life Sciences. Data were evaluated by taxonomic and rarefaction analyses. The 197,600 sequences generated yielded about 29,000 unique sequences, representing 22 taxonomic phyla. Grouping the sequences in operational taxonomic units (6%) yielded 3621 and 6888 species-level phylotypes in saliva and plaque, respectively. This work gives a radically new insight into the diversity of human oral microflora, which, with an estimated number of 19,000 phylotypes, is considerably higher than previously reported.
Subject
Healthy for Life
Biology
Healthy Living
Diversity
Microflora
Plaque
Pyrosequencing
Saliva
Bacterial DNA
Bacterial RNA
RNA 16S
Adult
Article
Bacterium identification
DNA sequence
Genetics
Human
Microbiology
Saliva
Tooth plaque
Adult
Bacterial Typing Techniques
Dental Plaque
DNA, Bacterial
Humans
RNA, Bacterial
RNA, Ribosomal, 16S
Saliva
Sequence Analysis, DNA
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DOI
https://doi.org/10.1177/154405910808701104
TNO identifier
241089
ISSN
0022-0345
Source
Journal of Dental Research, 87 (11), 1016-1020
Document type
article