Title
Apolipoprotein CI inhibits scavenger receptor BI and increases plasma HDL levels in vivo
Author
de Haan, W.
Out, R.
Berbée, J.F.P.
van der Hoogt, C.C.
Dijk, K.W.v.
van Berkel, T.J.C.
Romijn, J.A.
Wouter Jukema, J.
Havekes, L.M.
Rensen, P.C.N.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
Apolipoprotein CI (apoCI) has been suggested to influence HDL metabolism by activation of LCAT and inhibition of HL and CETP. However, the effect of apoCI on scavenger receptor BI (SR-BI)-mediated uptake of HDL-cholesteryl esters (CE), as well as the net effect of apoCI on HDL metabolism in vivo is unknown. Therefore, we evaluated the effect of apoCI on the SR-BI-mediated uptake of HDL-CE in vitro and determined the net effect of apoCI on HDL metabolism in mice. Enrichment of HDL with apoCI dose-dependently decreased the SR-BI-dependent association of [3H]CE-labeled HDL with primary murine hepatocytes, similar to the established SR-BI-inhibitors apoCIII and oxLDL. ApoCI deficiency in mice gene dose-dependently decreased HDL-cholesterol levels. Adenovirus-mediated expression of human apoCI in mice increased HDL levels at a low dose and increased the HDL particle size at higher doses. We conclude that apoCI is a novel inhibitor of SR-BI in vitro and increases HDL levels in vivo. © 2008 Elsevier Inc. All rights reserved.
Subject
Health
Physiological Sciences
High density lipoprotein
Oxidized LDL
SR-BI
Transgenic mice
cholesterol ester
high density lipoprotein cholesterol
scavenger receptor BI
Adenovirus
animal cell
cholesterol metabolism
dose response
in vivo study
liver cell
mouse
nonhuman
particle size
protein expression
Adenoviridae
Animals
Apolipoprotein C-I
Cholesterol, HDL
Gene Transfer Techniques
Hepatocytes
Humans
Mice
Mice, Knockout
Scavenger Receptors, Class B
Adenoviridae
Murinae
Mus
Mus musculus
To reference this document use:
http://resolver.tudelft.nl/uuid:9b69fd32-d47c-4fd5-9b5c-883ea34fa10c
DOI
https://doi.org/10.1016/j.bbrc.2008.10.147
TNO identifier
241309
ISSN
0006-291X
Source
Biochemical and Biophysical Research Communications, 377 (4), 1294-1298
Document type
article