Print Email Facebook Twitter The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro Title The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro Author van Nuenen, M.H.M.C. de Ligt, R.A.F. Doornbos, R.P. van der Woude, J.C.J. Kuipers, E.J. Venema, K. TNO Kwaliteit van Leven Publication year 2005 Abstract Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate, iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. Subject NutritionPhysiological SciencesInterleukin-10Intestinal epithelial cellsMacrophagesMicrobial metabolitesTumour necrosis factor-αammoniabutyric acidcytokineinterleukin 10tumor necrosis factor alphavaleric acidarticlecell strain CACO 2concentration (parameters)cytokine releaseelectric resistanceenteritisepithelium cellhumanhuman cellimmunitymacrophagemetabolismmetabolitephysiologypriority journalAmmonium ChlorideButyratesCaco-2 CellsEpithelial CellsHumansImmunity, MucosalInflammatory Bowel DiseasesInterleukin-10IntestinesMacrophagesPentanoic AcidsTumor Necrosis Factor-alphaU937 Cells To reference this document use: http://resolver.tudelft.nl/uuid:99a0a561-c893-4ad7-bf89-be4359974c98 DOI https://doi.org/10.1016/j.femsim.2005.03.010 TNO identifier 238626 ISSN 0928-8244 Source FEMS Immunology and Medical Microbiology, 45 (2), 183-189 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.