Title
The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro
Author
van Nuenen, M.H.M.C.
de Ligt, R.A.F.
Doornbos, R.P.
van der Woude, J.C.J.
Kuipers, E.J.
Venema, K.
TNO Kwaliteit van Leven
Publication year
2005
Abstract
Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate, iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Subject
Nutrition
Physiological Sciences
Interleukin-10
Intestinal epithelial cells
Macrophages
Microbial metabolites
Tumour necrosis factor-α
ammonia
butyric acid
cytokine
interleukin 10
tumor necrosis factor alpha
valeric acid
article
cell strain CACO 2
concentration (parameters)
cytokine release
electric resistance
enteritis
epithelium cell
human
human cell
immunity
macrophage
metabolism
metabolite
physiology
priority journal
Ammonium Chloride
Butyrates
Caco-2 Cells
Epithelial Cells
Humans
Immunity, Mucosal
Inflammatory Bowel Diseases
Interleukin-10
Intestines
Macrophages
Pentanoic Acids
Tumor Necrosis Factor-alpha
U937 Cells
To reference this document use:
http://resolver.tudelft.nl/uuid:99a0a561-c893-4ad7-bf89-be4359974c98
DOI
https://doi.org/10.1016/j.femsim.2005.03.010
TNO identifier
238626
ISSN
0928-8244
Source
FEMS Immunology and Medical Microbiology, 45 (2), 183-189
Document type
article