Title
Identification of outer membrane proteins of Yersinia pestis through biotinylation
Author
Smither, S.J.
Hill, J.
van Baar, B.L.M.
Hulst, A.G.
de Jong, A.L.
Titball, R.W.
TNO Defensie en Veiligheid
Publication year
2007
Abstract
The outer membrane of Gram-negative bacteria contains proteins that might be good targets for vaccines, antimicrobials or detection systems. The identification of surface located proteins using traditional methods is often difficult. Yersinia pestis, the causative agent of plague, was labelled with biotin. Tagged proteins were visualised through streptavidin probing of Western blots. Seven biotinylated proteins of Y. pestis were identified including two porins and the putative virulence factor catalase peroxidase. Crown Copyright © 2006.
Subject
Biotinylation
Outer membrane
Proteomics
Yersinia pestis
Bacterial enzyme
Bacterial protein
Biotin
Catalase
Catalase peroxidase
Outer membrane protein
Peroxidase
Streptavidin
Article
Bacterial virulence
Biotinylation
Nonhuman
Plague
Priority journal
Protein analysis
Proteomics
Western blotting
Yersinia pestis
Amino Acid Sequence
Bacterial Outer Membrane Proteins
Biotinylation
Blotting, Western
Electrophoresis, Gel, Two-Dimensional
Plague
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Yersinia pestis
Negibacteria
Yersinia pestis
Biotin, 58-85-5
Catalase, 9001-05-2
Peroxidase, 9003-99-0
Streptavidin, 9013-20-1
Bacterial Outer Membrane Proteins
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DOI
https://doi.org/10.1016/j.mimet.2006.05.014
TNO identifier
239815
ISSN
0167-7012
Source
Journal of Microbiological Methods, 68 (1 (January)), 26-31
Document type
article