Print Email Facebook Twitter Identification of outer membrane proteins of Yersinia pestis through biotinylation Title Identification of outer membrane proteins of Yersinia pestis through biotinylation Author Smither, S.J. Hill, J. van Baar, B.L.M. Hulst, A.G. de Jong, A.L. Titball, R.W. TNO Defensie en Veiligheid Publication year 2007 Abstract The outer membrane of Gram-negative bacteria contains proteins that might be good targets for vaccines, antimicrobials or detection systems. The identification of surface located proteins using traditional methods is often difficult. Yersinia pestis, the causative agent of plague, was labelled with biotin. Tagged proteins were visualised through streptavidin probing of Western blots. Seven biotinylated proteins of Y. pestis were identified including two porins and the putative virulence factor catalase peroxidase. Crown Copyright © 2006. Subject BiotinylationOuter membraneProteomicsYersinia pestisBacterial enzymeBacterial proteinBiotinCatalaseCatalase peroxidaseOuter membrane proteinPeroxidaseStreptavidinArticleBacterial virulenceBiotinylationNonhumanPlaguePriority journalProtein analysisProteomicsWestern blottingYersinia pestisAmino Acid SequenceBacterial Outer Membrane ProteinsBiotinylationBlotting, WesternElectrophoresis, Gel, Two-DimensionalPlagueSpectrometry, Mass, Electrospray IonizationSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationYersinia pestisNegibacteriaYersinia pestisBiotin, 58-85-5Catalase, 9001-05-2Peroxidase, 9003-99-0Streptavidin, 9013-20-1Bacterial Outer Membrane Proteins To reference this document use: http://resolver.tudelft.nl/uuid:97d4e568-e86d-41cc-a1de-0058271a8332 DOI https://doi.org/10.1016/j.mimet.2006.05.014 TNO identifier 239815 ISSN 0167-7012 Source Journal of Microbiological Methods, 68 (1 (January)), 26-31 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.