Title
Use of biosensors for rapid drug residue analysis without sample deconjugation or clean-up: A possible way forward
Author
Elliott, C.T.
Baxter, G.A.
Hewitt, S.A.
Arts, C.J.M.
van Baak, M.
Hellenäs, K.-E.
Johannson, A.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1998
Abstract
The drug salbutamol (SBL) is a β-agonist that may be used illegally as an animal growth promoter. SBL is also a good example of a drug which is excreted in the form of glucuronides and sulfates. Such metabolites cause complexities in analysing for the presence of drug residues. In the majority of cases a process of deconjugation and sample clean-up is required prior to analysis. This is both time consuming and causes some loss of accuracy. In this study, the urine of calves treated with SBL orally for 3 d was collected during and after medication. Samples were assayed before and after hydrolysis by two different methods, radioimmunoassay (RIA) and a newly developed biosensor immunoassay (BIA). Some samples were also analysed by GC-MS. The results clearly showed that both screening assays (RIA and BIA) found high concentrations of SBL residues throughout the study. This was especially true in the BIA method. It was also demonstrated that urine sample analysis without the need for deconjugation or clean-up could be achieved. Results obtained by GC-MS tended to be an order of magnitude lower than the corresponding screening test results. This work showed that biosensor based veterinary drug residue testing procedures can be developed which can generate results in real time without the need for time consuming sample preparation.
Subject
Nutrition
Drug residue
Reagent
Salbutamol
Solvent
Animal experiment
Biosensor
Cattle
Conference paper
Controlled study
Device
Drug conjugation
Drug determination
Drug hydrolysis
Drug urine level
Gas chromatography
Immunoassay
Male
Mass spectrometer
Mass spectrometry
Nonhuman
Oral drug administration
Radioimmunoassay
Adrenergic beta-Agonists
Albuterol
Animals
Biosensing Techniques
Cattle
Drug Residues
Gas Chromatography-Mass Spectrometry
Growth Substances
Immunoassay
Male
Radioimmunoassay
Sensitivity and Specificity
To reference this document use:
http://resolver.tudelft.nl/uuid:97c47175-5fed-499a-b393-239c657cb3c4
DOI
https://doi.org/10.1039/a804855j
TNO identifier
234793
ISSN
0003-2654
Source
Analyst, 123 (12), 2469-2473
Document type
article