Print Email Facebook Twitter Biochemical analysis of the articular cartilage and subchondral and trabecular bone of the metacarpophalangeal joint of horses with early osteoarthritis Title Biochemical analysis of the articular cartilage and subchondral and trabecular bone of the metacarpophalangeal joint of horses with early osteoarthritis Author van der Harst, M.R. de Groot, J. Kiers, G.H. Brama, P.A.J. van de Lest, C.H.A. van Weeren, P.R. Publication year 2005 Abstract Objective - To assess whether site-related changes in biochemical composition are present in the cartilage and subchondral and trabecular bone of the metacarpophalangeal joint of horses with early osteoarthritis. Sample population - Right metacarpophalangeal joints from 59 mature warmblood horses. Procedure - Biochemical data (cross-link, amino acid, DNA, and ash contents; denatured collagen and glycosaminoglycan [GAG] concentrations; bone mineral density; and mineral composition) were obtained from 2 differently loaded sites of phalanx I cartilage and subchondral and trabecular bone samples; data were compared with previously published values from nonosteoarthritic equine joints. Results - Compared with findings in nonosteoarthritic joints, GAG concentration was lower in cartilage from osteoarthritic joints and there was a loss of site differences in cellularity and lysylpyridinoline (LP) cross-link content. In subchondral bone, LP cross-link content was decreased overall and there was a loss of site differences in osteoarthritic joints; ash content was higher in the osteoarthritic joints. Hydroxyproline content in trabecular bone from osteoarthritic joints was greater than that in nonosteoarthritic trabecular bone. In all 3 layers and at both sites, the linear increase of the pentosidine cross-link content with age had diminished or was not apparent in the horses with osteoarthritic joints. Conclusions and clinical relevance - In equine metacarpophalangeal joints with early osteoarthritis, distinct biochemical changes were detected in the cartilage and subchondral and trabecular bone. The dissimilarity in response of the different tissues and differences between the sites that are affected may be related to differences in biomechanical loading and transmission and dissipation of force. Chemicals / CAS: amino acid, 65072-01-7; calcium, 7440-70-2; collagen, 9007-34-5; deoxypyridinoline, 83462-55-9; DNA, 9007-49-2; hydroxyproline, 51-35-4, 6912-67-0; pentosidine, 124505-87-9; phosphorus, 7723-14-0; Glycosaminoglycans Subject Amino acidCalciumCollagenDeoxypyridinolineDNAHydroxyprolinePentosidinePhosphorusAnimal tissueArticular cartilageAshBiochemistryBiomechanicsBone mineralConcentration (parameters)Controlled studyCross linkingMedical assessmentMetacarpophalangeal jointNonhumanPhalanxTrabecular boneWeight bearingAnimalsBone and BonesBone DensityCartilage, ArticularForelimbGlycosaminoglycansHorse DiseasesHorsesOsteoarthritisAnimaliaEquidaeEquus caballus To reference this document use: http://resolver.tudelft.nl/uuid:9750b15c-7c16-455b-8583-b2f983bbbe7f DOI https://doi.org/10.2460/ajvr.2005.66.1238 TNO identifier 238580 ISSN 0002-9645 Source American Journal of Veterinary Research, 66 (7), 1238-1246 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.