Title
An Advanced LC–MS/MS Platform for the Analysis of Specialized Pro-Resolving Lipid Mediators
Author
Jónasdóttir, H.S.
Ioan-Facsinay, A.
Kwekkeboom, J.
Brouwers, H.
Zuurmond, A.M.
Toes, R.
Deelder, A.M.
Giera, M.
Publication year
2014
Abstract
Here we present an advanced platform for the analysis of specialized pro-resolving mediators (SPM), including a set of pro-inflammatory lipids and respective biochemical pathway markers. The platform is characterized by a largely simplified sample preparation protocol, employing methanol protein-precipitation instead of solid phase extraction. Sample preparation and analysis can be carried out in 96-well format, ensuring higher throughput. In addition, faster analysis times and higher sensitivities have been achieved when compared to other platforms. In total 36 lipid mediators—SPM, their pathway markers and a set of pro-inflammatory lipids including three internal standards—are analyzed within an 11 min LC–MS/MS run. The method was validated using human plasma, including repeatability, linearity, recovery, matrix effects, and accuracy. The presented platform was applied in the analysis of synovial fluid post-mortem samples and cellular extracts from polymorphonuclear cells as well as whole blood treated with ionophore in the presence of the polyunsaturated fatty acid eicosapentaenoic acid. © 2014, Springer-Verlag Berlin Heidelberg.
Subject
Life
MHR - Metabolic Health Research
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biology
Healthy Living
EPA
Inflammation resolution
LC–MS
LTE4
Pro-resolving mediators
Cell extract
Icosapentaenoic acid
Ionophore
Lipid
Methanol
Polyunsaturated fatty acid
Specialized pro resolving lipid
Unclassified drug
Isolation and purification
Lipid analysis
Liquid chromatography
Mass spectrometry
Polymorphonuclear cell
Solid phase extraction
Synovial fluid
Validation study
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DOI
https://doi.org/10.1007/s10337-014-2779-5
TNO identifier
524069
ISSN
0009-5893
Source
Chromatographia, 78 (5-6), 391-401
Document type
article