Print Email Facebook Twitter Mass-spectrometry-based metabolomics: Limitations and recommendations for future progress with particular focus on nutrition research Title Mass-spectrometry-based metabolomics: Limitations and recommendations for future progress with particular focus on nutrition research Author Scalbert, A. Brennan, L. Fiehn, O. Hankemeier, T. Kristal, B.S. van Ommen, B. Pujos-Guillot, E. Verheij, E. Wishart, D. Wopereis, S. TNO Kwaliteit van Leven Publication year 2009 Abstract Mass spectrometry (MS) techniques, because of their sensitivity and selectivity, have become methods of choice to characterize the human metabolome and MS-based metabolomics is increasingly used to characterize the complex metabolic effects of nutrients or foods. However progress is still hampered by many unsolved problems and most notably the lack of well established and standardized methods or procedures, and the difficulties still met in the identification of the metabolites influenced by a given nutritional intervention. The purpose of this paper is to review the main obstacles limiting progress and to make recommendations to overcome them. Propositions are made to improve the mode of collection and preparation of biological samples, the coverage and quality of mass spectrometry analyses, the extraction and exploitation of the raw data, the identification of the metabolites and the biological interpretation of the results. © 2009 The Author(s). Subject BiologyAnalytical researchMass spectrometryMetabolomicsMethod developmentNutrition To reference this document use: http://resolver.tudelft.nl/uuid:916b37b7-751b-41f4-9cd3-743ab7de54a1 DOI https://doi.org/10.1007/s11306-009-0168-0 TNO identifier 281708 ISSN 1573-3882 Source Metabolomics, 5 (4), 435-458 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.