Title
Extracellular matrix components and regulators in the airway smooth muscle in asthma
Author
Araujo, B.B.
Dolhnikoff, M.
Silva, L.F.F.
Elliot, J.
Lindeman, J.H.N.
Ferreira, D.S.
Mulder, A.
Gomes, H.A.P.
Fernezlian, S.M.
James, A.
Mauad, T.
TNO Kwaliteit van Leven
Publication year
2008
Abstract
There is an intimate relationship between the extracellular matrix (ECM) and smooth muscle cells within the airways. Few studies have comprehensively assessed the composition of different ECM components and its regulators within the airway smooth muscle (ASM) in asthma. With the aid of image analysis, the fractional areas of total collagen and elastic fibres were quantified within the ASM of 35 subjects with fatal asthma (FA) and compared with 10 nonfatal asthma (NFA) patients and 22 nonasthmatic control cases. Expression of collagen I and III, fibronectin, versican, matrix metalloproteinase (MMP)-1, -2, -9 and -12 and tissue inhibitor of metalloproteinase-1 and -2 was quantified within the ASM in 22 FA and 10 control cases. In the large airways of FA cases, the fractional area of elastic fibres within the ASM was increased compared with NFA and controls. Similarly, fibronectin, MMP-9 and MMP-12 were increased within the ASM in large airways of FA cases compared with controls. Elastic fibres were increased in small airways in FA only in comparison with NFA cases. There is altered extracellular matrix composition and a degradative environment within the airway smooth muscle in fatal asthma patients, which may have important consequences for the mechanical and synthetic functions of airway smooth muscle. Copyright©ERS Journals Ltd 2008. Chemicals / CAS: collagen, 9007-34-5; fibronectin, 86088-83-7; gelatinase A, 146480-35-5; gelatinase B, 146480-36-6; interstitial collagenase, 9001-12-1; tissue inhibitor of metalloproteinase 1, 140208-24-8; tissue inhibitor of metalloproteinase 2, 124861-55-8; versican, 126968-45-4; tissue inhibitor of metalloproteinase, 97837-28-0; Matrix Metalloproteinases, EC 3.4.24.-; Tissue Inhibitor of Metalloproteinases
Subject
Airway smooth muscle
Extracellular matrix
Fatal asthma
Metalloproteinases
Remodelling
Tissue inhibitor of metalloproteinase
Adolescent
Adult
Asthma
Bronchi
Case-Control Studies
Extracellular Matrix
Female
Humans
Male
Matrix Metalloproteinases
Middle Aged
Muscle, Smooth
Tissue Inhibitor of Metalloproteinases
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DOI
https://doi.org/10.1183/09031936.00147807
TNO identifier
240901
ISSN
0903-1936
Source
European Respiratory Journal, 32 (1), 61-69
Document type
article