Title
Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens
Author
Jayasena, S.
Smits, M.
Fiechter, D.
de Jong, A.
Nordlee, J.
Baumert, J.
Taylor, S.L.
Pieters, R.H.
Koppelman, S.J.
Publication year
2015
Abstract
Six commercial peanut enzyme-linked immunosorbent assay kits were assessed for their ability to recover peanut from the standard reference material 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3, and Ara h 6. The percentage recovery of peanut from peanut butter differed across different kits as well as at different sample concentrations. The highest recovery was observed with the Romer and R-Biopharm kits, while four other kits were found to underestimate the protein content of the reference peanut butter samples. Five of the kits were most sensitive in detecting Ara h 3 followed by Ara h 1, while hardly recognizing Ara h 2 and Ara h 6. The other kit showed the highest sensitivity to Ara h 2 and Ara h 6, while Ara h 1 and Ara h 3 were poorly recognized. Although Ara h 2 and Ara h 6 are known to be heat stable and more potent allergens, antisera specific to any of these four peanut proteins/allergens may serve as good markers for the detection of peanut residues. © 2015 American Chemical Society.
Subject
Life
FI - Functional Ingredients
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biolog
Healthy Living
Detection
Allergens
Error detection
Food products
Proteins
Recovery
Ara h 1
Ara h 2
Ara h 3
Ara h 6
ELISA
Immunoassay
Peanut
Oilseeds
Ara
Arachis hypogaea
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http://resolver.tudelft.nl/uuid:8ce16bf6-5996-492f-a46f-d8363a037c1a
DOI
https://doi.org/10.1021/jf504741t
TNO identifier
524128
ISSN
0021-8561
Source
Journal of Agricultural and Food Chemistry, 63 (6), 1849-1855
Document type
article