Print Email Facebook Twitter β1 integrins regulate chondrogenesis and rock signaling in adipose stem cells Title β1 integrins regulate chondrogenesis and rock signaling in adipose stem cells Author Lu, Z.F. Doulabi, B.Z. Huang, C.L. Bank, R.A. Helder, M.N. TNO Kwaliteit van Leven Publication year 2008 Abstract β1 integrins play a controversial role during chondrogenesis. Since the maturation of chondrocytes relies on a signaling switch from cell-cell to cell-matrix interactions, we hypothesized that β1 integrins play a different role at the earlier (mainly cell-cell interaction) from the later stage (mainly cell-matrix interaction) of chondrogenesis. Our data showed: in plain medium, sox9, collagen X, and collagen II gene expressions of ASCs were induced by β1-integrin blockage at day 14. In chondrogenic medium, however, sox 9, sox6, and collagen II gene expression were induced at day 4 but inhibited at day 14. In addition, both β1-integrin blockage and TGF-β1 down-regulated Rock-1 and -2 gene expression and produced the round cells. We concluded that β1 integrins play a more important role at the later stages than earlier stages of chondrogenesis, and that the onset of chondrogenesis promoted by β1-integrin blockage might be through inhibiting Rock signaling. © 2008 Elsevier Inc. All rights reserved. Subject Healthy for LifeHealthHealthy LivingBiomedical Researchβ1 integrinsAdipose tissueCell shapeChondrogenesisMesenchymal stem cellsRock signalingbeta1 integrincollagen type 10collagen type 2transcription factor Sox9transforming growth factor beta1DNA binding proteinhigh mobility group proteinimmunoglobulin GRho kinasesox-9 transcription factorSOX6 protein, humantranscription factoradipocytearticlecell interactionchondrogenesiscollagen 2 genecollagen x genecontrolled studydown regulationextracellular matrixgenegene expressionhumanhuman cellhuman tissuepriority journalprotein functionrock 1 generock 2 genesignal transductionSox9 geneadipose tissuecell shapeculture mediumcytologydrug antagonismdrug effectgene expression regulationgeneticsphysiologystem cellAdipose TissueAntigens, CD29Cell ShapeChondrogenesisCollagen Type IICollagen Type XCulture MediaDNA-Binding ProteinsGene ExpressionGene Expression Regulation, DevelopmentalHigh Mobility Group ProteinsHumansImmunoglobulin Grho-Associated KinasesStem CellsTranscription FactorsTransforming Growth Factor beta1 To reference this document use: http://resolver.tudelft.nl/uuid:8cbe1cc4-b2b9-4511-8080-5b8b8204684c DOI https://doi.org/10.1016/j.bbrc.2008.05.063 TNO identifier 240949 ISSN 0006-291X Source Biochemical and Biophysical Research Communications, 372 (4), 547-552 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.