Print Email Facebook Twitter A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members Title A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members Author Agalou, A. Purwantomo, S. Övernäs, E. Johannesson, H. Zhu, X. Estiati, A. de Kam, R.J. Engström, P. Slamet-Loedin, I.H. Zhu, Z. Wang, M. Xiong, L. Meijer, A.H. Ouwerkerk, P.B.F. TNO Kwaliteit van Leven Publication year 2008 Abstract The homeodomain leucine zipper (HD-Zip) genes encode transcription factors that have diverse functions in plant development and have often been implicated in stress adaptation. The HD-Zip genes are the most abundant group of homeobox (HB) genes in plants and do not occur in other eukaryotes. This paper describes the complete annotation of the HD-Zip families I, II and III from rice and compares these gene families with Arabidopsis in a phylogeny reconstruction. Orthologous pairs of rice and Arabidopsis HD-Zip genes were predicted based on neighbour joining and maximum parsimony (MP) trees with support of conserved intron-exon organization. Additionally, a number of HD-Zip genes appeared to be unique to rice. Searching of EST and cDNA databases and expression analysis using RT-PCR showed that 30 out of 31 predicted rice HD-Zip genes are expressed. Most HD-Zip genes were broadly expressed in mature plants and seedlings, but others showed more organ specific patterns. Like in Arabidopsis and other dicots, a subset of the rice HD-Zip I and II genes was found to be regulated by drought stress. We identified both drought-induced and drought-repressed HD-Zip genes and demonstrate that these genes are differentially regulated in drought-sensitive versus drought-tolerant rice cultivars. The drought-repressed HD-Zip family I gene, Oshox4, was selected for promoter-GUS analysis, showing that drought-responsiveness of Oshox4 is controlled by the promoter and that Oshox4 expression is predominantly vascular-specific. Loss-of-function analysis of Oshox4 revealed no specific phenotype, but overexpression analysis suggested a role for Oshox4 in elongation and maturation processes. © 2007 The Author(s). Subject ArabidopsisDroughtHomeoboxHomeodomain-leucine zipperRiceCropsDroughtElongationPlants (botany)SeedTranscription factorsArabidopsisHomeoboxHomeodomain-leucine zipperRiceGenesarticlechromosome mapdisasterexpressed sequence taggeneticsgenomehomeoboxphylogenyreverse transcription polymerase chain reactionriceChromosome MappingExpressed Sequence TagsGenes, HomeoboxGenome, PlantNatural DisastersOryza sativaPhylogenyReverse Transcriptase Polymerase Chain ReactionArabidopsisDicotyledoneaeEukaryota To reference this document use: http://resolver.tudelft.nl/uuid:8ca1862d-c17b-4d94-a380-74d6ef47dd4b DOI https://doi.org/10.1007/s11103-007-9255-7 TNO identifier 240588 ISSN 0167-4412 Source Plant Molecular Biology, 66 (1-2), 87-103 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.