Print Email Facebook Twitter Influence of Bacillus subtilis C-3102 on microbiota in a dynamic in vitro model of the gastrointestinal tract simulating human conditions Title Influence of Bacillus subtilis C-3102 on microbiota in a dynamic in vitro model of the gastrointestinal tract simulating human conditions Author Hatanaka, M. Nakamura, Y. Maathuis, A.J.H. Venema, K. Murota, I. Yamamoto, N. Publication year 2012 Abstract Survival and germination rate of Bacillus subtilis C-3102 spores were investigated in a stomach and small intestine model (TIM-1), while the impact of C-3102 cells that had passed through TIM-1 on human colon microbiota was evaluated in a model of the large intestine (TIM-2). The survival of C-3102 spores in TIM-1 was 99%; 8% of the spores had germinated. Effluent of TIM-1 was subsequently introduced into TIM-2 and a micro-array platform was employed to assess changes in the microbiota composition. The effluent, which contained germinated C-3102 cells, increased some Bifidobacterium species and decreased some Clostridium groups. These changes were greater compared to those obtained by adding C-3102 spores directly to TIM-2. The present study suggests that oral doses of B. subtilis C-3102 spores have the potential to modulate the human colon microbiota. This effect may be caused by germination of the spores in the gastrointestinal tract. © 2012 Wageningen Academic Publishers. Subject LifePHS - Pharmacokinetics & Human StudiesEELS - Earth, Environmental and Life SciencesBiomedical InnovationHealthy LivingBacillus subtilisC-3102Gastrointestinal tract modelMicrobiotaProbiotic To reference this document use: http://resolver.tudelft.nl/uuid:89c46a35-86e4-41f8-a8fe-24d1420f7e38 DOI https://doi.org/10.3920/bm2012.0016 TNO identifier 466462 ISSN 1876-2883 Source Beneficial Microbes, 3 (3), 229-236 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.