Title
Physiological and phylogenetic characterization of a stable benzene-degrading, chlorate-reducing microbial community
Author
Weelink, S.A.B.
Tan, N.C.G.
ten Broeke, H.
van Doesburg, W.
Langenhoff, A.A.M.
Gerritse, J.
Stams, A.J.M.
TNO Bouw en Ondergrond
Publication year
2007
Abstract
A stable anoxic enrichment culture was obtained that degraded benzene with chlorate as an electron acceptor. The benzene degradation rate was 1.65 mM benzene per day, which is similar to reported aerobic benzene degradation rates but 20-1650 times higher than reported for anaerobic benzene degradation. Denaturing gradient gel electrophoresis of part of the 16S rRNA gene, cloning and sequencing showed that the culture had a stable composition after the seventh transfer. Five bacterial clones were further analyzed. Two clones corresponded to bacteria closely related to Alicycliphilus denitrificans K601. The three other clones corresponded to bacteria closely related to Zoogloea resiniphila PIV-3A2w, Mesorhizobium sp. WG and Stenotrophomonas acidaminiphila. DGGE analysis of cultures grown with different electron donors and acceptors indicated that the bacterium related to Alicycliphilus denitrificans K601 is able to degrade benzene coupled to chlorate reduction. The role of the other bacteria could not be conclusively determined. The bacterium related to Mesorhizobium sp. WG can be enriched with benzene and oxygen, but not with acetate and chlorate, while the bacterium related to Stenotrophomonas acidaminophila grows with acetate and chlorate, but not with benzene and oxygen. As oxygen is produced during chlorate reduction, an aerobic pathway of benzene degradation is most likely. © 2007 Federation of European Microbiological Societies.
Subject
Environment
Benzene degradation
Chlorate reduction
Enrichment culture
benzene
chlorate
anoxic conditions
bacterium
benzene
biodegradation
inorganic salt
microbial community
oxic conditions
phylogenetics
physiology
alicycliphilus denitrificans
anoxia
article
bacterial gene
bacterial growth
bacterial strain
bacterium culture
degradation kinetics
denaturing gradient gel electrophoresis
Mesorhizobium
microflora
molecular cloning
molecular phylogeny
molecular stability
nucleotide sequence
priority journal
sequence homology
stenotrophomonas acidaminiphila
zoogloea resiniphila
Bacteria
Benzene
Chlorates
DNA, Bacterial
Ecosystem
Electrophoresis
Molecular Sequence Data
Oxidation-Reduction
Phylogeny
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Alicycliphilus denitrificans
Bacteria (microorganisms)
Mesorhizobium sp. WG
Stenotrophomonas acidaminiphila
Zoogloea resiniphila
To reference this document use:
http://resolver.tudelft.nl/uuid:8675fd47-7c0f-495f-b28f-c90ae253cdb7
TNO identifier
239960
ISSN
0168-6496
Source
FEMS Microbiology Ecology, 60 (2), 312-321
Document type
article