Print Email Facebook Twitter Relationship between synovial fluid levels of glycosaminoglycans, hydroxyproline and general MMP activity and the presence and severity of articular cartilage change on the proximal articular surface of P1 Title Relationship between synovial fluid levels of glycosaminoglycans, hydroxyproline and general MMP activity and the presence and severity of articular cartilage change on the proximal articular surface of P1 Author TNO Preventie en Gezondheid van den Boom, R. van der Harst, M.R. Brommer, H. Brama, P.A.J. Barneveld, A. van Weeren, P.R. de Groot, J. Publication year 2005 Abstract Reasons for performing study: Osteoarthritis (OA) is one of the most prevalent and disabling chronic conditions affecting horses and leads to degeneration of articular cartilage. Diagnosis is based on clinical signs in combination with radiography, which is relatively insensitive and provides only an indication of accumulated damage. Alternative methods, such as molecular markers, are therefore needed that can quantitatively, reliably and sensitively detect osteoarthritic changes in the joints at an early stage of the disease. If such markers are to be used reliably, it is important to know the relationship between marker concentration and cartilage composition. Objectives: To study the relationship between cartilage composition, synovial fluid levels of glycosaminoglycans (GAGs), hydroxyproline (Hyp) and general matrix metalloproteinase (MMP) activity, and the presence and severity of articular cartilage damage on the articular surface of P1. Methods: Synovial fluid (SF) was collected from the metacarpophalangeal joints of 60 mature horses, and levels of GAGs, Hyp and general MMP activity were determined. Further, GAG and denatured collagen content of the articular cartilage were determined at the dorsal articular margin of P1 (site 1) and central cavity (site 2). The presence and severity of cartilage change was quantified using the cartilage degeneration index (CDI), measured at the same 2 sites. Correlations between SF parameters, cartilage composition and degree of cartilage degeneration were sought using correlation analysis. Results: There was no correlation between GAG or Hyp content of SF and the amount of GAGs or denatured collagen, respectively, in cartilage. In joints with moderate to severe cartilage damage, the GAG content of site 1 was significantly lower than in joints with no to minimal cartilage change (P = 0.005) and there was a negative correlation between the amount of denatured collagen and GAG content at site 1 in all joints (r = -039, P = 0.002). Further, in joints with moderate to severe cartilage damage, there was a significant positive correlation between MMP activity in SF and Hyp levels in SF (r = 0.72, P Subject BiomarkersGlycosaminoglycansHorseHydroxyprolineOsteoarthritisSynovial fluidcollagenglycosaminoglycanhydroxyprolinematrix metalloproteinaseanimal experimentanimal tissuearticular cartilagecartilage degenerationcontrolled studycorrelation analysisdisease severityenzyme activitymeasurementmetacarpophalangeal jointnonhumanquantitative analysissynovial fluidAge FactorsAnimalsBiological MarkersCartilage, ArticularGlycosaminoglycansHorse DiseasesHorsesHydroxyprolineMatrix MetalloproteinasesOsteoarthritisSeverity of Illness IndexSynovial FluidAnimaliaEquidaeEquus caballus To reference this document use: http://resolver.tudelft.nl/uuid:83c6b5a7-40f3-4693-850b-1a3cd160e4f3 DOI https://doi.org/10.2746/0425164054406919 TNO identifier 238295 ISSN 0425-1644 Source Equine Veterinary Journal, 37 (37), 19-25 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.