Title
Relationship between synovial fluid levels of glycosaminoglycans, hydroxyproline and general MMP activity and the presence and severity of articular cartilage change on the proximal articular surface of P1
Author
TNO Preventie en Gezondheid
van den Boom, R.
van der Harst, M.R.
Brommer, H.
Brama, P.A.J.
Barneveld, A.
van Weeren, P.R.
de Groot, J.
Publication year
2005
Abstract
Reasons for performing study: Osteoarthritis (OA) is one of the most prevalent and disabling chronic conditions affecting horses and leads to degeneration of articular cartilage. Diagnosis is based on clinical signs in combination with radiography, which is relatively insensitive and provides only an indication of accumulated damage. Alternative methods, such as molecular markers, are therefore needed that can quantitatively, reliably and sensitively detect osteoarthritic changes in the joints at an early stage of the disease. If such markers are to be used reliably, it is important to know the relationship between marker concentration and cartilage composition. Objectives: To study the relationship between cartilage composition, synovial fluid levels of glycosaminoglycans (GAGs), hydroxyproline (Hyp) and general matrix metalloproteinase (MMP) activity, and the presence and severity of articular cartilage damage on the articular surface of P1. Methods: Synovial fluid (SF) was collected from the metacarpophalangeal joints of 60 mature horses, and levels of GAGs, Hyp and general MMP activity were determined. Further, GAG and denatured collagen content of the articular cartilage were determined at the dorsal articular margin of P1 (site 1) and central cavity (site 2). The presence and severity of cartilage change was quantified using the cartilage degeneration index (CDI), measured at the same 2 sites. Correlations between SF parameters, cartilage composition and degree of cartilage degeneration were sought using correlation analysis. Results: There was no correlation between GAG or Hyp content of SF and the amount of GAGs or denatured collagen, respectively, in cartilage. In joints with moderate to severe cartilage damage, the GAG content of site 1 was significantly lower than in joints with no to minimal cartilage change (P = 0.005) and there was a negative correlation between the amount of denatured collagen and GAG content at site 1 in all joints (r = -039, P = 0.002). Further, in joints with moderate to severe cartilage damage, there was a significant positive correlation between MMP activity in SF and Hyp levels in SF (r = 0.72, P
Subject
Biomarkers
Glycosaminoglycans
Horse
Hydroxyproline
Osteoarthritis
Synovial fluid
collagen
glycosaminoglycan
hydroxyproline
matrix metalloproteinase
animal experiment
animal tissue
articular cartilage
cartilage degeneration
controlled study
correlation analysis
disease severity
enzyme activity
measurement
metacarpophalangeal joint
nonhuman
quantitative analysis
synovial fluid
Age Factors
Animals
Biological Markers
Cartilage, Articular
Glycosaminoglycans
Horse Diseases
Horses
Hydroxyproline
Matrix Metalloproteinases
Osteoarthritis
Severity of Illness Index
Synovial Fluid
Animalia
Equidae
Equus caballus
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DOI
https://doi.org/10.2746/0425164054406919
TNO identifier
238295
ISSN
0425-1644
Source
Equine Veterinary Journal, 37 (37), 19-25
Document type
article