Title
Identification and characterization of the furfural and 5-(hydroxymethyl)furfural degradation pathways of Cupriavidus basilensis HMF14
Author
Koopman, F.
Wierckx, N.
de Winde, J.H.
Ruijssenaars, H.J.
TNO Kwaliteit van Leven
Publication year
2010
Abstract
The toxic fermentation inhibitors in lignocellulosic hydrolysates pose significant problems for the production of second-generation biofuels and biochemicals. Among these inhibitors, 5-(hydroxymethyl) furfural (HMF) and furfural are specifically notorious. In this study, we describe the complete molecular identification and characterization of the pathway by which Cupriavidus basilensis HMF14 metabolizes HMF and furfural. The identification of this pathway enabled the construction of an HMF and furfural-metabolizing Pseudomonas putida. The genetic information obtained furthermore enabled us to predict the HMF and furfural degrading capabilities of sequenced bacterial species that had not previously been connected to furanic aldehyde metabolism. These results pave the way for in situ detoxification of lignocellulosic hydrolysates, which is a major step toward improved efficiency of utilization of lignocellulosic feedstock.
Subject
Biology
Degradation
Hydolysate
Hydroxymethyl furfural
Inhibitors
Lignocellulosic
5 (hydroxymethyl)furfural
aldehyde derivative
furan derivative
furfural
lignocellulose
protein hydrolysate
unclassified drug
article
bacterial gene
bacterial strain
bacterium identification
carbon source
catabolism
controlled study
Cupriavidus
Cupriavidus basilensis
detoxification
gene sequence
microbial degradation
nonhuman
nucleotide sequence
priority journal
Pseudomonas putida
Cupriavidus
DNA Transposable Elements
Furaldehyde
Genes, Bacterial
Metabolic Networks and Pathways
Mutagenesis
Oxidoreductases
Phenotype
Pseudomonas putida
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DOI
https://doi.org/10.1073/pnas.0913039107
TNO identifier
347462
ISSN
0027-8424
Source
Proceedings of the National Academy of Sciences of the United States of America, 107 (11), 4919-4924
Document type
article