Title
Bioconversion of red ginseng saponins in the gastro-intestinal tract in vitro model studied by high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry
Author
Kong, H.
Wang, M.
Venema, K.
Maathuis, A.
van der Heijden, R.
van der Greef, J.
Xu, G.
Hankemeier, T.
TNO Kwaliteit van Leven
Publication year
2009
Abstract
A high-performance liquid chromatography-high resolution Fourier transform ion cyclotron resonance mass spectrometry (HPLC-FTICR-MS) method was developed to investigate the metabolism of ginsenosides in in vitro models of the gastro-intestinal tract. The metabolites were identified by high-resolution tandem mass spectrometry. Degradation and bioconversion routes of the different ginsenosides at acidic (gastric) conditions and in the presence of intestinal microbiota were elaborated. Besides hydrolysis (deglycosylation) also hydration reactions occurred at acidic conditions. The results illustrate the value of metabolite profiling by HPLC-FTICR-MS for understanding of the mechanisms in bioavailability of herbal drugs and their metabolites. © 2008 Elsevier B.V. All rights reserved.
Subject
Biology
Analytical research
Biomedical research
Acidic hydrolysis
Ginsenoside
HPLC-FTICR-MS
Intestinal bacteria
Metabolism
Metabolite profiling
Panax ginseng
Gastro-intestinal model
TIM
Acidic hydrolysis
Ginsenoside
HPLC-FTICR-MS
Intestinal bacteria
Metabolite profiling
Panax ginseng
Bacteriology
Biochemistry
Biomolecules
Body fluids
Chromatographic analysis
Chromatography
Cyclotron resonance
Cyclotrons
Electron cyclotron resonance
High performance liquid chromatography
High pressure liquid chromatography
Hydrolysis
Ion chromatography
Liquid chromatography
Mass spectrometers
Mass spectrometry
Metabolism
Metabolites
Pharmacodynamics
Plasmas
Resonance
Spectrometers
Spectrometry
Spectrum analysis
Technological forecasting
Thermal insulating materials
Fourier transforms
Chinese medicine
Biological Availability
Biotransformation
Chromatography, High Pressure Liquid
Fourier Analysis
Ginsenosides
Humans
Hydrogen-Ion Concentration
Intestine, Large
Mass Spectrometry
Models, Biological
Panax
Reproducibility of Results
Solid Phase Extraction
Panax ginseng
To reference this document use:
http://resolver.tudelft.nl/uuid:74f3b8f1-dd32-43c2-86b1-8d11a0ecaaa0
DOI
https://doi.org/10.1016/j.chroma.2008.11.030
TNO identifier
241441
ISSN
0021-9673
Source
Journal of Chromatography A, 1216 (11), 2195-2203
Document type
article