Title
Experimental models of the gut microbiome
Author
Venema, K.
van den Abbeele, P.
Publication year
2013
Abstract
The human gut contains a diverse microbiota with large potential to influence health. Given the difficulty to access the main sites of the gut, in vitro models have been developed to dynamically monitor microbial processes at the site of metabolic activity. These models range from simple batch fermentations to complex multi-compartmental continuous systems. The latter include different models, focussing on similar but each also on distinct digestive parameters. The most intensively used include the three-stage continuous culture system, SHIME®, EnteroMix, Lacroix model and TIM-2. Especially after inclusion of surface-attached mucosal microbes (M-SHIME), such models have been shown representative of the in vivo situation in terms of microbial composition and activity. They have even been shown to maintain the interpersonal variation among different human fecal inocula. Novel developments, such as the incorporation of host cells, will further broaden the potential of in vitro models to unravel the importance of gut microbes for human health and disease. © 2013 Elsevier Ltd. All rights reserved.
Subject
Life
PHS - Pharmacokinetics & Human Studies
EELS - Earth, Environmental and Life Sciences
Biomedical Innovation
Biology
Healthy Living
Human gastrointestinal tract
In vitro model
Microbiome
Short-chain fatty acids
To reference this document use:
http://resolver.tudelft.nl/uuid:726f0b2d-3866-4529-8002-b77053c377ca
DOI
https://doi.org/10.1016/j.bpg.2013.03.002
TNO identifier
474206
ISSN
1521-6918
Source
Best Practice and Research: Clinical Gastroenterology, 27 (1), 115-126
Document type
article