Print Email Facebook Twitter Fibroblast-like synoviocyte-chondrocyte interaction in cartilage degradation Title Fibroblast-like synoviocyte-chondrocyte interaction in cartilage degradation Author Steenvoorden, M.M.C. Bank, R.A. Ronday, H.K. Toes, R.E.M. Huizinga, T.W.J. de Groot, J. TNO Kwaliteit van Leven Publication year 2007 Abstract Objective: In vitro models for joint diseases often focus on a single cell type, such as chondrocytes in osteoarthritis (OA) or fibroblast-like synoviocytes (synoviocytes) in rheumatoid arthritis (RA). However, these joint diseases affect the whole joint and interaction between chondrocytes and synoviocytes may play an important role in disease pathology. The current study was designed to study the use of the alginate recovered chondrocyte method as a model for cartilage degradation and to study interaction between chondrocytes and synoviocytes. Methods: Bovine chondrocytes were cultured in alginate beads for 1 week, subsequently chondrons were retrieved and seeded into transwells. Every two days cartilage-slices were analysed for proteoglycan content (colorimetric, Blyscan GAG kit), collagen content (HPLC) and collagen HP and LP crosslinking (HPLC). For degradation experiments, monocultures of cartilage-slices labelled with 35S and cocultures with synoviocytes were stimulated with IL-1β or TNF-α. After 7 days, 35S release was measured taken as a measure of cartilage degradation. Results: After biochemical analysis, three week old cartilage-like slices were chosen to perform cartilage-degradation experiments. Synoviocytes were able to induce cartilage degradation only in the presence of living chondrocytes. In addition, the cytokines interleukin 1 (IL-1β) and tumor necrosis factor (TNF-α) were only able to induce cartilage degradation by chondrocytes, not by synoviocytes. Conclusion: These data indicate that the alginate recovered chondrocyte method provides a novel model for cartilage degradation in which the interaction between synoviocytes and chondrocytes can be studied. © Copyright Clinical and Experimental Rheumatology 2007. Subject HealthBiomedical ResearchAlginate recovered chondrocytesCartilageCartilage degradationFibroblast-like synoviocytesIn vitro modelRheumatoid arthritisAlginatesAnimalsArthritis, RheumatoidCartilageCattleCell CommunicationCells, CulturedChondrocytesCollagenDisease Models, AnimalFibroblastsGlucuronic AcidHexuronic AcidsInterleukin-1betaOsteoarthritisProteoglycansSynovial MembraneTumor Necrosis Factor-alpha To reference this document use: http://resolver.tudelft.nl/uuid:6de56eef-a34e-479c-b4db-e0e1bd953706 TNO identifier 239858 ISSN 0392-856X Source Clinical and Experimental Rheumatology, 25 (2), 239-245 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.