Title
1H-NMR spectroscopy combined with pattern recognition analysis reveals characteristic chemical patterns in urines of MS patients and non-human primates with MS-like disease
Author
't Hart, B.A.
Vogels, J.T.W.E.
Spijksma, G.
Brok, H.P.M.
Polman, C.
van der Greef, J.
Primatencentrum TNO
Publication year
2003
Abstract
Proton nuclear magnetic resonance (1H-NMR) spectroscopy in combination with pattern recognition techniques were used to investigate the composition of organic compounds in urines from patients with multiple sclerosis (MS), patients with other neurological diseases (OND) and healthy controls (H). Using a valid animal model of MS, namely the common marmoset (Callithrix jacchus) model of experimental autoimmune encephalomyelitis (EAE), the relation of disease progression and alteration of the urine composition was investigated. Urine samples were collected during different stages of EAE, either induced with whole human myelin or with the myelin protein MOG in complete adjuvant. The urine samples were analysed with 1H-NMR spectroscopy allowing simultaneous detection of an array of compounds. Spectral differences between urines from EAE-affected and healthy monkeys were assessed with multivariate analysis. Evidence is provided that development of EAE is associated with changes in the chemical composition of the urine, in particular of compounds with NMR peaks in the region of the spectrum between 0.5 and 3.50 ppm. In addition, we found preliminary evidence for differences between urines from MS, OND and H groups. © 2003 Elsevier Science B.V. All rights reserved. Chemicals/CAS: tritium, 10028-17-8; Glycoproteins; Myelin Proteins; Tritium, 10028-17-8
Subject
Biology
Analytical research
Inflammatory demyelination
MS disease markers
NMR spectroscopy
Pattern recognition
Urine
myelin protein
organic compound
glycoprotein
tritium
animal model
article
autoimmune disease
chemical composition
clinical article
controlled study
disease course
encephalomyelitis
female
human
male
marmoset
molecular recognition
multiple sclerosis
multivariate analysis
nonhuman
primate
priority journal
proton nuclear magnetic resonance
urinalysis
validation process
allergic encephalomyelitis
animal
automated pattern recognition
chemistry
comparative study
disease model
immunization
immunology
methodology
nuclear magnetic resonance spectroscopy
species difference
time
urine
Animals
Callithrix
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental
Female
Glycoproteins
Humans
Immunization
Magnetic Resonance Spectroscopy
Male
Multiple Sclerosis
Multivariate Analysis
Myelin Proteins
Pattern Recognition, Automated
Species Specificity
Time Factors
Tritium
Urine
To reference this document use:
http://resolver.tudelft.nl/uuid:6dab44c8-2e31-4fe6-99d3-d27c600c3e08
DOI
https://doi.org/10.1016/s0022-510x(03)00080-7
TNO identifier
237225
ISSN
0022-510X
Source
Journal of the Neurological Sciences, 212 (1-2), 21-30
Document type
article