Print Email Facebook Twitter Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application Title Overproduction of the Aspergillus niger feruloyl esterase for pulp bleaching application Author Record, E. Asther, M. Sigoillot, C. Pagès, S. Punt, P.J. Delattre, M. Haon, M. van den Hondel, C.A.M.J.J. Sigoillot, J.C. Lesage-Meessen, L. Asther, M. TNO Voeding Publication year 2003 Abstract A well-known industrial fungus for enzyme production, Aspergillus niger, was selected to produce the feruloyl esterase FAEA by homologous overexpression for pulp bleaching application. The gpd gene promoter was used to drive FAEA expression. Changing the nature and concentration of the carbon source nature (maltose to glucose; from 2.5 to 60 g l-1), improved FAEA activity 24.5-fold and a yield of 1 g l-1 of the corresponding protein in the culture medium was achieved. The secreted FAEA was purified 3.5-fold to homogeneity in a two-step purification procedure with a recovery of 69%. The overproduced protein was characterised and presented properties in good agreement with those of native FAEA. The recombinant FAEA was tested for wheat straw pulp bleaching, with or without a laccase mediator system and xylanase. Best results were obtained using a bi-sequential process with a sequence including xylanase, FAEA and laccase, and yielded very efficient delignification - close to 75% - and a kappa number of 3.9. This is the first report on the potential application of recombinant FAEA in the pulp and paper sector. Chemicals/CAS: hemicellulose, 63100-39-0, 63100-40-3, 9034-32-6; laccase, 80498-15-3; xylan endo 1,3 beta xylosidase, 37278-89-0, 9025-55-2; Carboxylic Ester Hydrolases, EC 3.1.1.-; Endo-1,4-beta Xylanases, EC 184.108.40.206; feruloyl esterase, EC 220.127.116.11; Laccase, EC 18.104.22.168; Recombinant Proteins Subject BiologyBiotechnologyferuloyl esterasehemicelluloselaccaserecombinant enzymeunclassified drugxylan endo 1,3 beta xylosidasefunguspulp and paper industryarticleAspergillus nigerbleachingcarbon sourceculture mediumdelignificationenzyme activityenzyme purificationenzyme releaseenzyme synthesisgene overexpressionpaper industrypromoter regionprotein expressionpulp processingwheatAspergillus nigerBiotechnologyCarboxylic Ester HydrolasesEndo-1,4-beta XylanasesGenetic VectorsIndustryLaccasePaperRecombinant ProteinsTransformation, GeneticTriticumAspergillusAspergillus nigerFungiTriticum aestivum To reference this document use: http://resolver.tudelft.nl/uuid:6ce623c6-a055-4f09-9b8e-a30c3ee82fba DOI https://doi.org/10.1007/s00253-003-1325-4 TNO identifier 237252 ISSN 0175-7598 Source Applied Microbiology and Biotechnology, 62 (4), 349-355 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.