Title
Protein synthesis inhibition by cycloheximide does not affect the acute release of tissue-type plasminogen activator
Author
Tranquille, N.
Emeis, J.J.
Gaubius Instituut TNO
Publication year
1989
Abstract
The acute release of tissue-type plasminogen activator (t-PA) was studied in perfused rat hindlegs. Pretreatment of rats with the protein synthesis inhibitor cycloheximide (2 mg/kg) at 1, 3 or 5 h prior to perfusion of rat hindlegs did not influence the amount of t-PA released by platelet-activating factor (20 nM) or bradykinin (1 μM). The amount of t-PA activity that could be extracted from hindleg skeletal muscle was not decreased by cycloheximide pretreatment though it was decreased in lung extracts. The in vivo release of t-PA was not affected by cycloheximide pretreatment. The data suggest that the acute release of t-PA from vascular endothelial cells does not require ongoing protein synthesis, but that acutely released t-PA is derived from a stable endothelial storage pool. Chemicals/CAS: cycloheximide, 642-81-9, 66-81-9; plasminogen activator, 9039-53-6; Cycloheximide, 66-81-9; Protein Synthesis Inhibitors; Tissue Plasminogen Activator, EC 3.4.21.68
Subject
Health
cycloheximide
plasminogen activator
animal cell
enzyme inhibition
nonhuman
Animal
Cycloheximide
Fibrinolysis
Hindlimb
Lung
Male
Muscles
Perfusion
Protein Synthesis Inhibitors
Rats
Rats, Inbred Strains
Tissue Plasminogen Activator
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TNO identifier
230886
ISSN
0340-6245
Source
Thrombosis and Haemostasis, 61 (3), 442-447
Document type
article