Title
TrgI, toluene repressed gene I, a novel gene involved in toluene-tolerance in Pseudomonas putida S12
Author
Volkers, R.J.M.
Ballerstedt, H.
Ruijssenaars, H.
de Bont, J.A.M.
de Winde, J.H.
Wery, J.
TNO Kwaliteit van Leven
Publication year
2009
Abstract
Pseudomonas putida S12 is well known for its remarkable solvent tolerance. Transcriptomics analysis of this bacterium grown in toluene-containing chemostats revealed the differential expression of 253 genes. As expected, the genes encoding one of the major solvent tolerance mechanisms, the solvent efflux pump SrpABC and its regulatory genes srpRS were heavily up-regulated. The increased energy demand brought about by toluene stress was also reflected in transcriptional changes: genes involved in sugar storage were down-regulated whereas genes involved in energy generation such as isocitrate dehydrogenase and NADH dehydrogenases, were up-regulated in the presence of toluene. Several flagella-related genes were up-regulated and a large group of transport genes were down-regulated. In addition, a novel Pseudomonas-specific gene was identified to be involved in toluene tolerance of P. putida S12. This toluene-repressed gene, trgI, was heavily down-regulated immediately upon toluene exposure in batch cultures. The relationship of trgI with solvent tolerance was confirmed by the increased resistance to toluene shock and toluene induced lysis of trgI knock-out mutants. We propose that down-regulation of trgI plays a role in the first line of defence against solvents. © 2008 Springer.
Subject
Biology
Biotechnology
Pseudomonas putida S12
Solvent-tolerance
Toluene
Transcriptomics
TrgI
bacterial protein
solvent
toluene
article
bacterial gene
biosynthesis
chemistry
DNA microarray
drug effect
flagellum
gene expression regulation
genetic transcription
genetics
metabolism
polymerase chain reaction
principal component analysis
Pseudomonas putida
time
Bacterial Proteins
Flagella
Gene Expression Regulation, Bacterial
Genes, Bacterial
Oligonucleotide Array Sequence Analysis
Polymerase Chain Reaction
Principal Component Analysis
Pseudomonas putida
Solvents
Time Factors
Toluene
Transcription, Genetic
Bacteria (microorganisms)
Pseudomonas
Pseudomonas putida
To reference this document use:
http://resolver.tudelft.nl/uuid:655eb00e-6479-429b-821d-470129c4e6d4
DOI
https://doi.org/10.1007/s00792-008-0216-0
TNO identifier
241429
ISSN
1431-0651
Source
Extremophiles, 13 (2), 283-297
Document type
article