Title
Aerosolized endotoxin is immediately bound by pulmonary surfactant protein D in vivo
Author
van Rozendaal, B.A.W.M.
van de Lest, C.H.A.
van Eijk, M.
van Golde, L.M.G.
Voorhout, W.F.
van Helden, H.P.M.
Haagsman, H.P.
Publication year
1999
Abstract
Collectins are carbohydrate binding proteins that are implicated in innate host defense. The lung collectins, surfactant proteins A and D (SP-A and SP-D), bind a variety of pathogens in vitro and influence phagocytosis by alveolar macrophages. In this report we show that SP-D binds endotoxin (lipopolysaccharide, LPS) in vivo in a rat model of acute respiratory distress syndrome (ARDS). Intratracheal aerosolization of LPS in rats resulted in the typical features of human ARDS. Total amounts of SP-D, as well as the carbohydrate binding properties of SP-D were measured in lung lavage as a function of time. The amount of SP-D did not change during 24 h. Interestingly, SP-D in lung lavage isolated from rats during the first 2 h after LPS treatment, was not able to bind to carbohydrate. Further analysis revealed that the carbohydrate binding sites of SP-D were occupied by LPS, suggesting that SP-D is an LPS scavenging molecule in vivo. Electron microscopic analysis indicated that, 1 h after LPS aerosolization, aggregates of SP-D with LPS were found in lysosomal structures in alveolar macrophages. We conclude that the lung collectin SP-D binds inhaled endotoxin in vivo, which may help to protect the lung from endotoxin-induced disease. Copyright (C) 1999 Elsevier Science B.V.
Subject
Health
Alveolar macrophage
Collectin
Host defense
Lipopolysaccharide
Lung
Surfactant protein D (SP-D)
Carbohydrate binding protein
Endotoxins
Lipopolysaccharide
Surfactant protein a
Surfactant protein d
Animal model
Animal tissue
Binding site
Controlled study
Electron microscopy
Enzyme linked immunosorbent assay
Lung lavage
Male
Nonhuman
Protein analysis
Protein binding
Respiratory distress
Aerosols
Animals
Bronchoalveolar lavage vluid
Disease Models
Animal
Dose-Response Relationship, Drug
Glycoproteins
Histocytochemistry
Lipopolysaccharides
Macrophages, Alveolar
Mannans
Pulmonary Surfactant-Associated Protein D
Pulmonary Surfactants
Rats
Rats, Wistar
Respiratory Distress Syndrome, Adult
Time Factors
Trachea
Fluorescein-5-isothiocyanate, 3326-32-7
Glycoproteins
Lipopolysaccharides
To reference this document use:
http://resolver.tudelft.nl/uuid:63ac89ed-2d58-4963-b081-a238ec792988
DOI
https://doi.org/10.1016/s0925-4439(99)00042-3
TNO identifier
235133
ISSN
0925-4439
Source
Biochimica et Biophysica Acta - Molecular Basis of Disease, 1454 (3), 261-269
Document type
article