Title
A new method to measure iron absorption from the enrichment of 57Fe and 58Fe in young erythroid cells
Author
van den Heuvel, E.G.H.M.
Muys, T.
Pellegrom, H.
Bruyntjes, J.P.
van Dokkum, W.
Spanhaak, S.
Schaafsma, G.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1998
Abstract
Iron absorption can be measured by the incorporation of stable iron isotopes into erythrocytes, 14 days after isotope administration. The disadvantage of this method is the high dose of isotopes needed to obtain a sufficient enrichment. Therefore, in this study cell fractions rich in young erythroid cells were prepared by using a density separation method. From 10 women blood was taken 4, 5, and 7 days after oral and intravenous administration of 57Fe and 58Fe. In these cell fractions and in whole blood taken 14 days after isotope administration, isotope enrichment was measured and absorption calculated. Absorption calculated from the isotope enrichment in the reticulocyte-rich cell fractions (12.2 ± SEM 3.7%) was not significantly different from absorption based on higher isotope enrichment was found in the cell fractions, the required dose of stable isotopes can be reduced to one-third of the dose used in the traditional method without loss of sensitivity.
Subject
Nutrition
Iron
Adult
Cell membrane transport
Controlled study
Erythroid cell
Female
Human
Human cell
Iron absorption
Iron kinetics
Isotope labeling
Normal human
Radioisotope distribution
Absorption
Administration, Oral
Adult
Cell Separation
Centrifugation, Zonal
Erythrocyte Aging
Erythrocytes
Female
Ferritins
Humans
Injections, Intravenous
Iron
Iron Isotopes
Isotope Labeling
Mass Spectrometry
Reticulocytes
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TNO identifier
234430
ISSN
0009-9147
Source
Clinical Chemistry, 44 (3), 649-654
Document type
article