Print Email Facebook Twitter Soluble fibrin species in arterial thrombi Title Soluble fibrin species in arterial thrombi Author Sandbæk, G. Bjørnsen, S. Sobel, J.H. Nieuwenhuizen, W. Matsueda, G. Brosstad, F. Gaubius Instituut TNO Publication year 2000 Abstract The aim of this study was to characterize soluble fibrin(ogen) species in human, arterial, in-vivo-formed thrombi, using the immunoblotting technique. Specimens were collected via intra-arterial catheters in six patients scheduled for catheter-directed thrombolysis. Unreduced and reduced samples of the supernatants from the arterial thrombi-derived specimens were electrophoresed on polyacrylamide gels and immunoblotted, using specific mono- and polyclonal anti-fibrin(ogen) antibodies. The reduced samples disclosed substantial amounts of high molecular weight material, consistent with α-chain polymers and γγ-dimers, as well as lower molecular weight material, such as α-, β- and γ-chains. No fibrinogen with intact fibrinopeptide A was detectable, and des-AABB fibrin represented a major fibrin derivative in the soluble part of the arterial thrombi. The α-chains were C-terminally degraded, most of them distal to position 259. In conclusion, we have demonstrated the presence of cross-linked fibrin derivatives in soluble, arterial thrombus-related material, without signs of fibrinogen-fibrin hybrids. The fibrin derivatives were C-terminally degraded, thus representing X-oligomeric material, most probably originating from plasmin degradation of insoluble thrombus fibrin. The present study supports the hypothesis of a dynamic equilibrium between clotting and lysis in thrombi. (C) 2000 Lippincott Williams and Wilkins. Chemicals/CAS: Antibodies, Monoclonal; Fibrin, 9001-31-4; Fibrinogen, 9001-32-5; Fibrinopeptide A, 25422-31-5 Subject AgedAntibodies, MonoclonalArteriesBlotting, WesternFemaleFibrinFibrinogenFibrinopeptide AHumansMaleMiddle AgedMolecular WeightOxidation-ReductionProtein Structure, QuaternarySolubilityThrombosis To reference this document use: http://resolver.tudelft.nl/uuid:601815ea-8769-4611-a217-faa7ee7687b0 TNO identifier 235450 ISSN 0957-5235 Source Blood Coagulation and Fibrinolysis, 11 (1), 1-5 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.