Title
High-performance liquid chromatographic determination of urinary free catecholamines with electrochemical detection after prepurification on immobilized boric acid
Author
Speek, A.J.
Odink, J.
Schrijver, J.
Schreurs, W.H.P.
Centraal Instituut voor Voedingsonderzoek TNO
Publication year
1983
Abstract
A reliable high-performance liquid chromatographic method is presented for the determination of the urinary free catecholamines noradrenaline, adrenaline and dopamine. Urine is purified on a column of immobilized boric acid. Catecholamines are separated by ion-pair reversed phase high-performance liquid chromatography and detected electrochemically. The method is suited for routine analysis. It allows the determination of urinary free catecholamines in concentrations as low as 1 ??g/l for noradrenaline and adrenaline and 5 ??g/l for dopamine. A single analysis can be completed within 1 h. Routine analyses can be carried out in a series of 40 samples within 2 days. The within-assay and between-assay coefficients of variation of the analyses in urine were both 2.9% for noradrenaline, both 5.0% for adrenaline, and 1.9 and 2.1% for dopamine. The chromatographic properties of the immobilized boric acid were investigated. In particular, the elution pattern of a series of catecholamine metabolites and analogues was determined. Under the conditions used, only basic compounds containing both a vicinal hydroxyl configuration and a primary or secondary amino group adsorb and elute together with the free catecholamines. Chemicals/CAS: adrenalin, 51-43-4, 55-31-2, 6912-68-1; alpha methyldopamine, 555-64-6; dopamine, 51-61-6, 62-31-7; isoprenaline, 299-95-6, 51-30-9, 6700-39-6, 7683-59-2; noradrenalin, 1407-84-7, 51-41-2; boric acid, 11113-50-1; Boric Acids; Catecholamines
Subject
Adrenalin
Alpha methyldopamine
Catecholamine
Dopamine
Isoprenaline
Noradrenalin
Drug analysis
Drug determination
Drug urine level
Liquid chromatography
Nonhuman
Theoretical study
Wine
Boric Acids
Catecholamines
Chromatography, High Pressure Liquid
Electrochemistry
Human
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DOI
https://doi.org/10.1016/0009-8981(83)90060-8
TNO identifier
229369
ISSN
0009-8981
Source
Clinica Chimica Acta, 128 (1), 103-113
Document type
article