Email Facebook Twitter Human osteoblasts produce cathepsin K Title Human osteoblasts produce cathepsin K Author Mandelin, J. Hukkanen, M. Li, T.F. Korhonen, M. Liljeström, M. Sillat, T. Hanemaaijer, R. Salo, J. Santavirta, S. Konttinen, Y.T. TNO Kwaliteit van Leven Publication year 2006 Abstract Healthy bone is a rigid yet living tissue that undergoes continuous remodeling. Osteoclasts resorb bone in the remodeling cycle. They secrete H+-ions and proteinases to dissolve bone mineral and degrade organic bone matrix, respectively. One of the main collagenolytic proteinase in osteoclasts is cathepsin K, a member of papain family cysteine proteinases. Recently, it has been shown that osteoblasts may contribute to organic matrix remodeling. We therefore investigated their ability to produce cathepsin K for this action. Trabecular bone samples were collected from patients operated due to a fracture of the femoral neck. Part of the bone was decalcified and the rest was used for cell isolation. Sections from the decalcified bone were immunostained with antibodies against cathepsin K. Isolated cells were characterized for their ability to form mineralized matrix and subsequently analyzed for their cathepsin K production by Western blotting and quantitative RT-PCR. Osteoblasts, bone lining cells and some osteocytes in situ showed cathepsin K immunoreactivity and osteoblast-like cells in vitro produced cathepsin K mRNA and released both 42 kDa pro- and 27 kDa processed cathepsin K to culture media. Osteoblastic cathepsin K may thus contribute to collagenous matrix maintenance and recycling of improperly processed collagen I. Whether osteoblastic cathepsin K synthesis has consequences in diseases characterized by abnormal bone matrix turnover remains to be investigated. © 2006. Chemicals / CAS: cathepsin K, 94716-09-3; Alkaline Phosphatase, EC 18.104.22.168; cathepsin K, EC 3.4.22.-; Cathepsins, EC 3.4.-; Core Binding Factor Alpha 1 Subunit; Matrix Metalloproteinase 13, EC 3.4.24.-; Osteoprotegerin; RANK Ligand; RNA, Messenger; RUNX2 protein, human; TNFSF11 protein, human; Tumor Necrosis Factor-alpha Subject Biomedical ResearchCathepsin KCytokinesOsteoblastsOsteoporosisStromal cellsantibodycathepsin Kcollagen type 1messenger RNAbone matrixcell isolationcontrolled studyculture mediumfemur neckfracturehumanhuman cellhuman tissueimmunoassayimmunohistochemistryimmunoreactivitynucleotide sequenceosteoblastosteocyteprotein analysisprotein functionprotein secretionprotein synthesisquantitative analysisreverse transcription polymerase chain reactionRNA analysissamplingtrabecular boneWestern blottingAlkaline PhosphataseCalcification, PhysiologicCathepsinsCell DifferentiationCells, CulturedCore Binding Factor Alpha 1 SubunitGene Expression RegulationHumansImmunohistochemistryMatrix Metalloproteinase 13Mesenchymal Stem CellsOsteoclastsOsteoprotegerinRANK LigandRNA, MessengerTumor Necrosis Factor-alpha To reference this document use: http://resolver.tudelft.nl/uuid:5afa6ee7-4bab-4513-9232-aadbf85420bf DOI https://doi.org/10.1016/j.bone.2005.10.017 TNO identifier 239276 ISSN 8756-3282 Source Bone, 38 (6), 769-777 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.