Print Email Facebook Twitter LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone Title LPS-induced IL-10 production in whole blood cultures from chronic fatigue syndrome patients is increased but supersensitive to inhibition by dexamethasone Author Visser, J. Graffelman, W. Blauw, B. Haspels, I. Lentjes, E. de Kloet, R. Nagelkerken, L. TNO Preventie en Gezondheid Publication year 2001 Abstract Several causes have been held responsible for the chronic fatigue syndrome (CFS), including an altered hypothalamus-pituitary-adrenal gland (HPA)-axis activity, viral infections and a reduced Th1 activity. Therefore, it was investigated whether the regulation of IL-10 is different in CFS. LPS-induced cytokine secretion in whole blood cultures showed a significant increase in IL-10 and a trend towards a decrease in IL-12 as compared with healthy controls. In patients and controls, IL-12 secretion was equally sensitive to suppression by dexamethasone, whereas IL-10 secretion appeared more sensitive in CFS-patients. In controls, IL-10 and IL-12 secretion were inversely correlated with free serum cortisol (r=-0.492, p<0.02 and r=-0.434, p<0.05, respectively). In CFS, such an inverse correlation was found for IL-12 (r=-0.611, p<0.02) but not for IL-10 (r=-0.341, ns). These data are suggestive for a disturbed glucocorticoid regulation of IL-10 in CFS. © 2001 Elsevier Science B.V. All rights reserved. Chemicals/CAS: Dexamethasone, 50-02-2; Glucocorticoids; Hydrocortisone, 50-23-7; Interleukin-10, 130068-27-8; Interleukin-12, 187348-17-0; Lipopolysaccharides; RNA, Messenger; Tumor Necrosis Factor-alpha Subject Chronic fatigue syndromeCortisolDexamethasoneGlucocorticoidsIL-10IL-12TNF-αViral IL-10Bacterium lipopolysaccharidBlood cultureChemosensitivityChronic fatigue syndromeControlled studyCorrelation functionCytokine productionCytokine releaseDrug blood levelDrug effectFemaleHypothalamus hypophysis systemImmunoregulationMajor clinical studyMaleAdolescentAdultCells, CulturedDexamethasoneFatigue Syndrome, ChronicGene ExpressionGlucocorticoidsHumansHydrocortisoneInterleukin-10Interleukin-12LeukocytesLipopolysaccharidesMiddle AgedRNA, MessengerTumor Necrosis Factor-alpha To reference this document use: http://resolver.tudelft.nl/uuid:5470142c-fd6a-4fe6-a2e3-dc9f7722189b DOI https://doi.org/10.1016/s0165-5728(01)00400-3 TNO identifier 236239 ISSN 0165-5728 Source Journal of Neuroimmunology, 119 (2), 343-349 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.