Title
Impact of galacto-oligosaccharides on the gut microbiota composition and metabolic activity upon antibiotic treatment during in vitro fermentation
Author
Ladirat, S.E.
Schuren, F.H.J.
Schoterman, M.H.C.
Nauta, A.
Gruppen, H.
Schols, H.A.
Publication year
2014
Abstract
Prebiotics are considered to have potential to reduce disturbances in the gut microbiota induced by antibiotics. Results in literature are, however, not consistent. The current in vitro study conducted in a fermentation screening platform allowed to unambiguously compare the impact of galacto-oligosaccharides (GOS) on adult gut microbiota composition and activity upon treatment with four antibiotics at two doses. The changes in relative abundance of bacteria upon antibiotic treatment and the growth of Bifidobacterium and Lactobacillus upon GOS addition were antibiotic and dose dependant. This conclusion explains discrepancies in literature and indicates that particular combinations of GOS antibiotic should be studied. The combination GOS-Amoxicillin was especially of interest as, after decrease in Bifidobacterium levels, a recovery of mainly Bifidobacterium longum was observed and could be correlated with specific degradation patterns of GOS. Next to different degradation profiles of individual GOS, an accumulation of monosaccharides and intermediate organic acids was observed in antibiotic-treated microbiota as compared to nontreated microbiota. © 2013 Federation of European Microbiological Societies.
Subject
Life
MSB - Microbiology and Systems Biology
ELSS - Earth, Life and Social Sciences
Biomedical Innovation
Biology
Healthy Living
Amoxicillin
Ciprofloxacin
Clindamycin
Doxycycline
Phylogenetic microarray
Prebiotic
To reference this document use:
http://resolver.tudelft.nl/uuid:5394427e-16e9-488b-811a-c1304b1d2be5
DOI
https://doi.org/10.1111/1574-6941.12187
TNO identifier
487297
ISSN
0168-6496
Source
FEMS Microbiology Ecology, 87 (1), 41-51
Document type
article