Title
Characterization of antibacterial polyethersulfone membranes using the respiration activity monitoring system (RAMOS)
Author
Kochan, J.
Scheidle, M.
van Erkel, J.
Bikel, M.
Büchs, J.
Wong, J.E.
Melin, T.
Wessling, M.
Publication year
2012
Abstract
Membranes with antibacterial properties were developed using surface modification of polyethersulfone ultrafiltration membranes. Three different modification strategies using polyelectrolyte layer-by-layer (LbL) technique are described. The first strategy relying on the intrinsic antibacterial properties of poly(diallyldimethylammonium chloride) (PDADMAC) and poly(ethylenimine) (PEI) exhibits only little antibacterial effects. The other two strategies contain silver in both ionic (Agþ) and metallic (Ag0) form. Agþ embedded into negatively charged poly(sodium 4-styrene sulfonate) (PSS) layers totally inhibits bacterial growth. Ag0 nanoparticles were introduced to the membrane surface by LbL deposition of chitosan- and poly(methacrylic acid) e sodium salt (PMA)-capped silver nanoparticles and subsequent UV or heat treatment. Antibacterial properties of the modified membranes were quantified by a new method based on the Respiration Activity Monitoring System (RAMOS), whereby the oxygen transfer rates (OTR) of E. coli K12 cultures on the membranes were monitored online. As opposed to colony forming counting method RAMOS yields more quantitative and reliable data on the antibacterial effect of membrane modification. Ag-imprinted polyelectrolyte film composed of chitosan (Ag0)/PMA(Ag0)/chitosan(Ag0) was found to be the most promising among the tested membranes. Further investigation revealed that the concentration and equal distribution of silver in the membrane surface plays an important role in bacterial growth inhibition
Subject
Earth & Environment
WT - Water Treatment
EELS - Earth, Environmental and Life Sciences
Geological Survey Netherlands
Geosciences
Energy / Geological Survey Netherlands
Antibacterial membranes
E. coli
Layer-by-layer technique
Respiration activity monitoring system (RAMOS)
Silver nanoparticles
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http://resolver.tudelft.nl/uuid:538bddda-4ee6-4e0c-ab69-187fcae3874b
TNO identifier
464255
ISSN
0043-1354
Source
Water Research, 46 (16), 5401-5409
Document type
article