Print Email Facebook Twitter Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A Title Enzymatic degradation of granular potato starch by Microbacterium aurum strain B8.A Author Sarian, F.D. van der Kaaij, R.M. Kralj, S. Wijbenga, D.-J. Binnema, D.J. van der Maarel, M.J.E.C. Dijkhuizen, L. Publication year 2012 Abstract Microbacterium aurum strain B8.A was isolated from the sludge of a potato starch-processing factory on the basis of its ability to use granular starch as carbon- and energy source. Extracellular enzymes hydrolyzing granular starch were detected in the growth medium of M. aurum B8.A, while the type strain M. aurum DSMZ 8600 produced very little amylase activity, and hence was unable to degrade granular starch. The strain B8.A extracellular enzyme fraction degraded wheat, tapioca and potato starch at 37 °C, well below the gelatinization temperature of these starches. Starch granules of potato were hydrolyzed more slowly than of wheat and tapioca, probably due to structural differences and/or surface area effects. Partial hydrolysis of starch granules by extracellular enzymes of strain B8.A resulted in large holes of irregular sizes in case of wheat and tapioca and many smaller pores of relatively homogeneous size in case of potato. The strain B8.A extracellular amylolytic system produced mainly maltotriose and maltose from both granular and soluble starch substrates; also, larger maltooligosaccharides were formed after growth of strain B8.A in rich medium. Zymogram analysis confirmed that a different set of amylolytic enzymes was present depending on the growth conditions of M. aurum B8.A. Some of these enzymes could be partly purified by binding to starch granules. © 2011 The Author(s). Subject LifeFI - Functional IngredientsEELS - Earth, Environmental and Life SciencesHealthy LivingAlpha-amylaseGranular starchMicrobacterium aurumPotato starchScanning electron microscopy To reference this document use: http://resolver.tudelft.nl/uuid:498dc864-cc33-4c59-9f9a-f369419f8a7e DOI https://doi.org/10.1007/s00253-011-3436- TNO identifier 446664 ISSN 0175-7598 Source Applied Microbiology and Biotechnology, 93 (2), 645-654 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.