Print Email Facebook Twitter The presence of two S-layer-protein-encoding genes is conserved among species related to Lactobacillus acidophilus Title The presence of two S-layer-protein-encoding genes is conserved among species related to Lactobacillus acidophilus Author Boot, H.J. Kolen, C.P.A.M. Pot, B. Kersters, K. Pouwels, P.H. Prins Maurits Laboratorium TNO Publication year 1996 Abstract Previously we have shown that the type strain of Lactobacillus acidophilus possesses two S-protein-encoding genes, one of which is silent, on a chromosomal segment of 6 kb. The S-protein-encoding gene in the expression site can be exchanged for the silent S-protein-encoding gene by inversion of this slp segment. In this study the presence of S-protein and corresponding S-protein-encoding genes of strains belonging to species that are closely related to L. acidophilus was determined. All strains investigated were identified by numerical comparison of highly standardized one-dimensional SDS-PAGE whole-cellular-protein patterns. Western blot and Southern blot methods were used to identify the presence of, and homology between, S-proteins and S-protein encoding genes. From these analyses we conclude that strains of L. acidophilus, L. crispatus, L. amylovorus and L. gallinarum possess an S-layer and contain two slp genes. Strains of L. helveticus possess an S-layer but have only one intact slp gene. Strains of L. gasseri, L. johnsonii and L. delbrueckii subsp. bulgaricus have neither an S-layer nor S-protein-encoding genes hybridizing with probes derived from the L. acidophilus slpA or slpB region. The presence of a highly conserved 5' region in the slp genes of strains of L. acidophilus, L. crispatus, L. amylovorus and L. gallinarum suggests that S-layer variation is a common feature for strains of these species. Subject BiologyS-layer variationS-proteinSDS-PAGE identificationSurface layerBacterial proteinBacterial geneChromosome inversionGenetic conservationImmunoblottingNonhumanPolyacrylamide gel electrophoresisPriority journalSilent geneSouthern blottingBacterial ProteinsBlotting, SouthernBlotting, WesternChromosome MappingElectrophoresis, Polyacrylamide GelMembrane ProteinsSequence Homology, Amino AcidSequence Homology, Nucleic AcidBacteria (microorganisms)LactobacillusLactobacillus acidophilusLactobacillus amylovorusLactobacillus crispatusLactobacillus delbrueckii subsp. bulgaricusLactobacillus gallinarumLactobacillus gasseriLactobacillus helveticusLactobacillus johnsonii To reference this document use: http://resolver.tudelft.nl/uuid:491a0f5e-9984-4d0a-bdf7-78a93e9351a2 TNO identifier 233434 ISSN 1350-0872 Source Microbiology, 142 (9), 2375-2384 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.