Print Email Facebook Twitter The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi Title The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi Author Joosten, V. Lokman, C. van den Hondel, C.A.M.J.J. Punt, P.J. TNO Voeding Publication year 2003 Abstract In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody fragment production (scFv and VHH) by these lower eukaryotes and the possible applications of these proteins. Also the coupling of fragments to relevant enzymes or other components will be discussed. As an example of the fusion protein strategy, the 'magic bullet' approach for industrial applications, will be highlighted. © 2003 Joosten et al; licensee BioMed Central Ltd. Subject BiologyBiotechnologycancer antibodycell antibodyfungus antibodyhybrid proteinimmunoglobulinimmunoglobulin F(ab) fragmentimmunoglobulin Fc fragmentimmunoglobulin heavy chainimmunoglobulin light chainintrabodyrecombinant antibodysingle chain fragment variable antibodyunclassified drugantibody combining siteantibody productionantigen bindingAspergillus awamoriCandida boidiniiclinical trialdrug clearancedrug delivery systemEscherichia colieukaryotegene expression systemHansenula polymorphahumanimmunotherapyKluyveromycesmethylotrophic bacteriummycologynonhumanPichiaPichia methanolicaPichia pastorisprotein protein interactionprotein synthesisreviewSaccharomyces cerevisiaeSchizosaccharomyces pombeTrichoderma reeseiYarrowia lipolyticaEukaryotaFungi To reference this document use: http://resolver.tudelft.nl/uuid:47de520f-fb56-4432-a4b1-4f891e52dfd8 DOI https://doi.org/10.1186/1475-2859-2-1 TNO identifier 236944 ISSN 1475-2859 Source Microbial Cell Factories, 2 Article number No.: 1 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.