Title
Effect of eugenol on the genotoxicity of established mutagens in the liver
Author
Rompelberg, C.J.M.
Evertz, S.J.C.J.
Bruijntjes-Rozier, G.C.D.M.
van den Heuvel, P.D.
Verhagen, H.
TNO Voeding
Publication year
1996
Abstract
The influence of in vivo treatment with eugenol on established mutagens was studied to determine whether eugenol has antigenotoxic potential. The effects of eugenol in rats was investigated in the unscheduled DNA synthesis (UDS) assay with established mutagens and the Salmonella typhimurium mutagenicity assay. In addition, the effect of in vitro treatment with eugenol on benzo[a]pyrene (B[a]P)-induced genotoxicity in human hepatoma cell line Hep G2 was investigated in the single-cell gel electrophoresis assay. The mutagenicity of B[a]P in the S. typhimurium mutagenicity assay was lower in liver S-9 fractions prepared from rats treated with eugenol orally (1000 mg/kg body weight) than in liver S-9 fractions from control rats. Incubation of liver S-9 fractions from eugenol-treated rats with dimethylbenzanthracene (DMBA) had no antimutagenic effect. Eugenol did not modify UDS activity in hepatocytes isolated from rats pretreated with eugenol orally after exposure of these cells in vitro to DMBA and aflatoxin B1. Four different treatment schemes of combinations of B[a]P and eugenol were examined in Hep G2 cells: pre-treatment with eugenol; simultaneous treatment with eugenol and B[a]P; a combination of these (pretreatment/simultaneous treatment); and post-treatment with eugenol. An increase in the genotoxicity of B[a]P was found in Hep G2 cells. No effect of eugenol on the genotoxicity of B[a]P was found with the pre- and post-treatments. It is concluded that the effect of eugenol on genotoxicity induced by established mutagens is not univocal: in vivo treatment of rats with eugenol resulted in a reduction of the mutagenicity of B[a]P in the S. typhimurium mutagenicity assay, while in the UDS assay no effect of eugenol was found. In vitro treatment of cultured cells with eugenol resulted in an increase in genotoxicity of B[a]P. These findings indicate that there is only limited support for the antigenotoxic potential of eugenol in vivo.
Subject
Toxicology
aflatoxin b1
benzo[a]pyrene
dimethylbenz[a]anthracene
eugenol
mutagenic agent
animal experiment
animal tissue
article
controlled study
genotoxicity
hepatoma cell
human
human cell
liver
male
metabolic activation
mutagenicity
nonhuman
oral drug administration
rat
salmonella typhimurium
unscheduled dna synthesis
9,10-Dimethyl-1,2-benzanthracene
Aflatoxin B1
Animals
Antimutagenic Agents
Benzo(a)pyrene
Carcinoma, Hepatocellular
Cytochrome P-450 Enzyme System
DNA
Electrophoresis, Agar Gel
Eugenol
Glutathione Transferase
Humans
Liver
Liver Neoplasms
Male
Micronucleus Tests
Mutagenicity Tests
Mutagens
Rats
Rats, Wistar
Salmonella typhimurium
Tumor Cells, Cultured
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DOI
https://doi.org/10.1016/0278-6915(95)00091-7
TNO identifier
233219
ISSN
0278-6915
Source
Food and Chemical Toxicology, 34 (1), 33-42
Document type
article