Print Email Facebook Twitter Challenges and advances in systems biology analysis of Bacillus spore physiology; molecular differences between an extreme heat resistant spore forming Bacillus subtilis food isolate and a laboratory strain Title Challenges and advances in systems biology analysis of Bacillus spore physiology; molecular differences between an extreme heat resistant spore forming Bacillus subtilis food isolate and a laboratory strain Author Brul, S. van Beilen, J. Caspers, M. O'Brien, A. de Koster, C. Oomes, S. Smelt, J. Kort, R. ter Beek, A. Publication year 2011 Abstract Bacterial spore formers are prime organisms of concern in the food industry. Spores from the genus Bacillus are extremely stress resistant, most notably exemplified by high thermotolerance. This sometimes allows surviving spores to germinate and grow out to vegetative cells causing food spoilage and possible intoxication. Similar issues though more pending toward spore toxigenicity are observed for the anaerobic Clostridia. The paper indicates the nature of stress resistance and highlights contemporary molecular approaches to analyze the mechanistic basis of it in Bacilli. A molecular comparison between a laboratory strain and a food borne isolate, very similar at the genomic level to the laboratory strain but generating extremely heat resistant spores, is discussed. The approaches cover genome-wide genotyping, proteomics and genome-wide expression analyses studies. The analyses aim at gathering sufficient molecular information to be able to put together an initial framework for dynamic modelling of spore germination and outgrowth behaviour. Such emerging models should be developed both at the population and at the single spore level. Tools and challenges in achieving the latter are succinctly discussed. © 2010 Elsevier Ltd. Subject LifeMSB - Microbiology and Systems BiologyEELS - Earth, Environmental and Life SciencesNutritionBacillusBacterial spore formersGenomicsGermination and outgrowthProteomicsSpores To reference this document use: http://resolver.tudelft.nl/uuid:44850fbb-2a26-45ac-8b36-03446a895e08 DOI https://doi.org/10.1016/j.fm.2010.06.011 TNO identifier 427539 ISSN 0740-0020 Source Food Microbiology, 28 (2), 221-227 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.