Title
Flexible biochips for detection of biomolecules
Author
Peter, M.
Schüler, T.
Furthner, F.
Rensing, P.A.
van Heck, G.T.
Schoo, H.F.M.
Möllier, R.
Fritzsche, W.
van Breemen, A.J.J.M.
Meinders, E.R.
TNO Industrie en Techniek
Publication year
2009
Abstract
Miniaturization of biosensors is envisaged by the development of biochips consisting of parallel microarray patterns of binding sites on rigid substrates, such as glass or silicon. Thin plastic substrates are promising flexible alternatives because of the possibility for large-area roll-to-roll manufacturing of disposable chips at lower costs. Mature optical lithography technology faces many challenges when used to pattern flexible foils as a result of the substrate instabilities, especially at higher temperatures. In this work, flexible biochips with gold electrode patterns were fabricated on thin polyethylene naphthalate (PEN) foils using photolithography. The gold electrode structures of the chips were manufactured by direct metal patterning and by lift-off processing. Both methodologies resulted in well-defined electrode patterns as concluded from optical microscopy and scanning electron microscopy (SEM) characterization and resistance measurements. The biochips were successfully employed for the electrical and optical detection of DNA molecules. The DNA detection was based on the immobilization of capture DNA between electrode gaps, hybridization with biotin-labeled target DNA, and enzymatic silver enhancement. © 2009 American Chemical Society.
Subject
Detection of biomolecules
DNA detection
DNA molecules
Electrode gap
Electrode pattern
Gold electrodes
Higher temperatures
Lower cost
Metal patterning
Optical detection
Optical lithography
Plastic substrates
Polyethylene naphthalate
Resistance measurement
Rigid substrates
Roll to roll manufacturing
SEM
Silver enhancement
Binding energy
Binding sites
Biochips
Biosensors
DNA
Genes
Nucleic acids
Optical microscopy
Photolithography
Scanning electron microscopy
Silver
Substrates
Bioassay
article
DNA microarray
genetic procedures
methodology
scanning electron microscopy
Biosensing Techniques
Microscopy, Electron, Scanning
Oligonucleotide Array Sequence Analysis
To reference this document use:
http://resolver.tudelft.nl/uuid:4178339c-f759-42b6-9a31-c7411a40c9e2
DOI
https://doi.org/10.1021/la8037457
TNO identifier
279990
ISSN
0743-7463
Source
Langmuir, 25 (9), 5384-5390
Document type
article