Title
Evaluation of the intestinal absorption of deoxynivalenol and nivalenol by an in vitro gastrointestinal model, and the binding efficacy of activated carbon and other adsorbent materials
Author
Avantaggiato, G.
Havenaar, R.
Visconti, A.
TNO Voeding
Publication year
2004
Abstract
In vitro screening of 14 adsorbent materials, including some commercial products used to detoxify Fusarium-mycotoxins, were tested in the pH range of 3-8 for deoxynivalenol (DON)- and nivalenol (NIV)-binding ability. Only activated carbon showed to be effective with binding capacities of 35.1 μmol and 8.8 μmol DON and NIV/g adsorbent, respectively, calculated from the adsorption isotherms. A dynamic laboratory model simulating the gastrointestinal (GI) tract of healthy pigs (TIM system) was used to evaluate the small-intestinal absorption of DON and NIV and the efficacy of activated carbon in reducing the relevant absorption. The in vitro intestinal absorptions of DON and NIV were 51% and 21%, respectively, as referred to 170 μg DON and 230 μg NIV ingested through contaminated (spiked) wheat. Most absorption occurred in the jejunal compartment for both mycotoxins. The inclusion of activated carbon produced a significant reduction in the intestinal mycotoxin absorption. At 2% inclusion level the absorption with respect to the intake was lowered from 51% to 28% for DON and from 21% to 12% for NIV. The binding activity of activated carbon for these trichothecenes was lower than that observed for zearalenone, a mycotoxin frequently co-occurring with them in naturally contaminated cereals. © 2004 Elsevier Ltd. All rights reserved.
Subject
Biology Health
Physiological Sciences
Activated carbon
Deoxynivalenol
DON
Gas chromatography
Gastrointestinal
GC
GI
High performance liquid chromatography
HPLC
Mycotoxin detoxification
NIV
Nivalenol
TIM
Gastrointestinal model
ZEA
Zearalenone
activated carbon
adsorbent
mycotoxin
nivalenol
trichothecene derivative
vomitoxin
zearalenone
animal experiment
animal tissue
article
binding affinity
calculation
commercial phenomena
controlled study
detoxification
dynamics
evaluation
food contamination
Fusarium
gastrointestinal tract
in vitro study
intestine absorption
isotherm
jejunum
model
nonhuman
pH
screening
simulation
small intestine absorption
swine
wheat
Adsorption
Animals
Charcoal
Food Contamination
Hydrogen-Ion Concentration
Intestinal Absorption
Intestine, Small
Models, Biological
Swine
Trichothecenes
Fusarium
Triticum aestivum
To reference this document use:
http://resolver.tudelft.nl/uuid:4057ea83-3c76-4fd3-a765-0bbef1b16926
DOI
https://doi.org/10.1016/j.fct.2004.01.004
TNO identifier
237726
ISSN
0278-6915
Source
Food and Chemical Toxicology, 42 (5), 817-824
Document type
article