Title
Probing the proteome response to toluene exposure in the solvent tolerant pseudomonas putida S12
Author
Wijte, D.
van Baar, B.L.M.
Heck, A.J.R.
Altelaar, A.F.M.
Publication year
2011
Abstract
To enhance target production from biocatalysts, it is necessary to thoroughly understand the molecular mechanisms involved in production, degradation, and, importantly, adaptation to the required environment. One such bacterium with high potential for biocatalysis is the solvent-tolerant bacteria Pseudomonas putida S12, which, among others, is able to degrade organic solvents. For bioconversion of organic solvents to become a successful industrial process, the understanding of the molecular response upon solvent tolerance is essential. Here we performed a quantitative analysis of the P. putida S12 proteome at different stages of adaptation to toluene. Using a stable isotope dimethylation labeling approach we monitored the differential expression of 528 proteins, including often hard-to-detect membrane associate proteins, such as multiple RND-family transporters and ABC transporters of nutrients. Our quantitative proteomics approach revealed the remarkable ability of P. putida S12 to severely change its protein expression profile upon toluene exposure. This proteome response entails a significant increase in energy metabolism and expression of the solvent efflux pump SrpABC, confirming its role in solvent tolerance. Other proteins strongly up-regulated in the presence of toluene include the multidrug efflux membrane protein PP1272 and the cation/acetate symporter ActP and may form interesting alternative targets for improving solvent tolerance. © 2011 American Chemical Society.
Subject
Life
CBRN - CBRN Protection
EELS - Earth, Environmental and Life Sciences
Biocatalysis
Efflux pumps
Organic solvent tolerance
P. putida S12
Quantitative proteomics
Stable isotope dimethyl labeling
Toluene
ABC transporter
Acetate symporter
Cotransporter
Isotope
Membrane protein
Organic solvent
Toluene
Unclassified drug
Article
Biocatalysis
Biotransformation
Controlled study
Degradation kinetics
Demethylation
Energy metabolism
Molecular probe
Nonhuman
Nutrient
Priority journal
Protein expression
Proteomics
Pseudomonas putida
Quantitative analysis
Upregulation
Chemicals/CAS: toluene, 108-88-3
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DOI
https://doi.org/10.1021/pr100401n
TNO identifier
429751
ISSN
1535-3893
Source
Journal of Proteome Research, 10 (2), 394-403
Document type
article