Title
The solvent-tolerant Pseudomonas putida S12 as host for the production of cinnamic acid from glucose
Author
Nijkamp, K.
van Luijk, N.
de Bont, J.A.M.
Wery, J.
TNO Kwaliteit van Leven TNO Voeding
Publication year
2005
Abstract
A Pseudomonas putida S12 strain was constructed that efficiently produced thefine chemical cinnamic acid from glucose or glycerol via the central metabolite phenylalanine. The gene encoding phenylalanine ammonia lyase from the yeast Rhodosporidium toruloides was introduced. Phenylalanine availability was the main bottleneck in cinnamic acid production, which could not be overcome by the overexpressing enzymes of the phenylalanine biosynthesis pathway. A successful approach in abolishing this limitation was the generation of a bank of random mutants and selection on the toxic phenylalanine anti-metabolite m-fluoro-phenylalanine. Following high-throughput screening, a mutant strain was obtained that, under optimised culture conditions, accumulated over 5 mM of cinnamic acid with a yield (Cmol%) of 6.7%. © Springer-Verlag 2005.
Subject
Nutrition
Biotechnology
Biosynthesis
Enzymes
Genes
Glucose
Glycerol
Metabolism
Organic acids
Toxic materials
Cinnamic acid
Phenylalanine ammonia lyase
Pseudomonas putida
Rhodosporidium toruloides
Strain
3 fluorophenylalanine
Antimetabolite
Cinnamic acid
Glucose
Glycerol
Phenylalanine
Phenylalanine ammonia lyase
Solvent
Amino acid synthesis
Bacterial strain
Bacterium culture
Bacterium mutant
Bioaccumulation
Bioavailability
Controlled study
Gene construct
Gene overexpression
Genetic code
High throughput screening
Host
Metabolite
Nonhuman
Pseudomonas putida
Quantum yield
Randomization
Rhodosporidium toruloides
Yeast
3-Deoxy-7-Phosphoheptulonate Synthase
Catalysis
Cinnamates
Drug Tolerance
Fermentation
Genetic Engineering
Genetic Vectors
Glucose
Mutation
Phenylalanine
Phenylalanine Ammonia-Lyase
Prephenate Dehydratase
Pseudomonas putida
Solvents
Bacteria (microorganisms)
Pseudomonas putida
Rhodosporidium toruloides
To reference this document use:
http://resolver.tudelft.nl/uuid:38519b59-0716-4d6d-bc4f-934525b3d8b3
DOI
https://doi.org/10.1007/s00253-005-1973-7
TNO identifier
238792
ISSN
0175-7598
Source
Applied Microbiology and Biotechnology, 69 (2), 170-177
Document type
article