Print Email Facebook Twitter Assessment of the allergic potential of food protein extracts and proteins on oral application using the Brown Norway rat model Title Assessment of the allergic potential of food protein extracts and proteins on oral application using the Brown Norway rat model Author Knippels, L.M.J. Penninks, A.H. TNO Voeding Publication year 2003 Abstract The need for widely accepted and validated animal models to test the potential allergenicity and potency of novel (biotechnology-derived) proteins has become an important issue for their safety evaluation.In this article, we summarize the results of the development of an oral sensitization protocol for food proteins in the rat. Young Brown Norway rats were exposed to either various purified allergenic proteins (e.g., ovalbumin, partly purified), a whole food (cow's milk), or total protein extracts (hen's egg white, peanut) by daily gavage dosing during 42 days without the use of an adjuvant. The results showed that Brown Norway rats can be sensitized orally to the various allergenic food proteins tested, resulting in antigen-specific immunoglobulin (Ig) G and IgE responses, without the use of adjuvants. Animals orally exposed to cow's milk or total protein extracts of egg white also developed specific IgE and IgG antibodies that recognized the same proteins compared with antibodies from patients allergic to egg white or cow's milk. We also studied local and systemic immune-mediated effects. In ovalbumin-sensitized rats, some clinical symptoms of food allergy were studied upon an oral challenge with ovalbumin. The results demostrated that gut permeability was increased and that in some animals breathing frequency and systolic blood pressure were temporarily decreased. The results obtained show that the Brown Norway rat provides a suitable model for food allergy research and for the study of relative allergenicity of existing and novel food proteins. Subject ToxicologyToxicology and Applied PharmacologyAllergic potentialBrown Norway ratsChallenge effectsEgg-white proteinsFood allergyFood proteinsIgEMilk proteinsOral sensitizationOvalbuminPassive cutaneous anaphylaxisadjuvantegg whitefood allergenimmunoglobulin Eimmunoglobulin Govalbuminallergenicityanimal experimentanimal modelantibody responseantigen specificitybreathing ratecontrolled studyfeedingfood allergyimmune responseintestine mucosa permeabilitymalemilk allergynonhumanpeanut allergypriority journalprotein intakeprotein purificationprovocation testratrat strainreviewrisk assessmentsensitizationsymptomatologysystolic blood pressureAdministration, OralAllergensAnimalsDisease Models, AnimalEgg ProteinsFood HypersensitivityHumansImmunoglobulin EImmunoglobulin GMaleMilk ProteinsRatsAnimaliaArachis hypogaeaRattus norvegicus To reference this document use: http://resolver.tudelft.nl/uuid:34a42064-050e-4646-9f39-821e7b4e5ce3 TNO identifier 236953 ISSN 0091-6765 Source Environmental Health Perspectives, 111 (2), 233-238 Document type article Files To receive the publication files, please send an e-mail request to TNO Library.